Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,901,121	A→G	D202D (GAT→GAC)	pstB ←	phosphate ABC transporter ATP‑binding protein PstB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,901,121	0	A	G	100.0%	52.6 / NA	18	D202D (GAT→GAC)	pstB	phosphate ABC transporter ATP‑binding protein PstB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/9);  total (9/9)

TCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTC  >  NZ_CP009273/3901041‑3901192                                                                                 |                                                                        tCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTc                                                                <  1:357255/90‑1 (MQ=255)   gCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAg                                                              <  1:369886/90‑1 (MQ=255)        ggTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCgg                                                         >  2:284090/1‑90 (MQ=255)                      gtgtGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCa                                           >  2:215550/1‑90 (MQ=255)                      gtgtGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGAAGCCCTGCTTCAGTTCGGGGATCAGCTCTTCa                                           >  1:93763/1‑90 (MQ=255)                             cGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGcc                                    >  2:37653/1‑90 (MQ=255)                              ggAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGccc                                   <  2:25495/90‑1 (MQ=255)                               gAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCCCCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCg                                  <  2:305751/90‑1 (MQ=255)                                aacaacGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCgg                                 >  1:363832/1‑90 (MQ=255)                                aacaacGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCgg                                 >  1:51103/1‑90 (MQ=255)                                  caacGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTa                               <  2:100245/90‑1 (MQ=255)                                  caacGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTa                               <  2:327971/90‑1 (MQ=255)                                    ccgcgCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTa                               >  2:374775/2‑88 (MQ=255)                                    ccgcgCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTa                               <  1:374775/87‑1 (MQ=255)                                                  gcATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAgcg               <  2:363832/90‑1 (MQ=255)                                                               gACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTtc  >  1:79763/1‑90 (MQ=255)                                                                            gtgtAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGcc                                    <  1:53623/43‑1 (MQ=255)                                                                            gtgtAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGcc                                    >  2:53623/1‑43 (MQ=255)                                                                                 |                                                                        TCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTC  >  NZ_CP009273/3901041‑3901192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑