Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,153,816	T→C	S84P (TCA→CCA)	btuB →	TonB‑dependent vitamin B12 receptor BtuB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,153,816	0	T	C	100.0%	51.9 / NA	18	S84P (TCA→CCA)	btuB	TonB‑dependent vitamin B12 receptor BtuB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/10);  total (8/10)

ACCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCTCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGT  >  NZ_CP009273/4153744‑4153899                                                                         |                                                                                    aCCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTcgc                                                                    <  2:395184/90‑1 (MQ=255)  ccTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTcgcg                                                                   <  2:37469/90‑1 (MQ=255)           atgatgTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATg                                                          >  1:64542/1‑90 (MQ=255)             gatgTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGcc                                                        <  1:202153/90‑1 (MQ=255)             gatgTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGcc                                                        <  2:357831/90‑1 (MQ=255)                gtgCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGGTTAGGTCAGATCCCATCTATTTTTTTTTCCGGGACAAAAGCCaag                                                     >  1:400408/1‑88 (MQ=255)                 tgctgcGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTc                                                    >  1:216303/1‑90 (MQ=255)                 tgctgcGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTc                                                    >  1:70842/1‑90 (MQ=255)                     gcgcCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGGACAAATTCCCGTCAtgt                                                >  1:96354/1‑90 (MQ=255)                         cGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCGTCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTg                                            <  2:161742/90‑1 (MQ=255)                          gTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTgg                                           >  1:176897/1‑90 (MQ=255)                             ttCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGgtgt                                        <  2:41887/90‑1 (MQ=255)                                    cGTCGATATCACCCAAAACGGCGGTTCAGGGCAGCTCCCCTCTTTTTTTTTTTTCGGGAAAAAAGCCCGGCCTGTGTTGGTGTTAAttta                                 >  2:368507/1‑88 (MQ=255)                                                        gcggTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGGACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGGACGCCTGAATCTgg             >  1:293278/1‑90 (MQ=255)                                                           gTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCgg          <  2:351943/90‑1 (MQ=255)                                                           gTTCAGGTCAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCgg          <  1:179724/90‑1 (MQ=255)                                                                   cAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGt  <  1:332836/90‑1 (MQ=255)                                                                   cAGCTCCCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGt  <  2:400408/90‑1 (MQ=255)                                                                         |                                                                                    ACCTCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCAGGTCAGCTCTCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGT  >  NZ_CP009273/4153744‑4153899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑