Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	609,731	A→G	D40G (GAT→GGT)	entF →	enterobactin non‑ribosomal peptide synthetase EntF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	609,731	0	A	G	100.0%	20.3 / NA	8	D40G (GAT→GGT)	entF	enterobactin non‑ribosomal peptide synthetase EntF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/3);  total (5/3)

GCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGATTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGA  >  NZ_CP009273/609645‑609815                                                                                       |                                                                                     gCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGGTTc                                                                                   <  2:413769/90‑1 (MQ=255)    cGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGGTTCGcc                                                                                <  1:14669/90‑1 (MQ=255)                   aGAAAAACTGTCAGAATTTCCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGGTTCGCCATTACTGGCCcgcgc                                                                 <  1:336384/90‑1 (MQ=255)                                          ccGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGGTTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAg                                          >  2:347636/1‑90 (MQ=255)                                                     gTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGGTTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTg                               >  2:74156/1‑90 (MQ=255)                                                             tcaCGTTGAGTTAACCGGAGAGGTTGGTTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCg                       >  2:218291/3‑90 (MQ=255)                                                                         aaCCGGAGAGGTTGGTTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGa           >  1:1525/1‑90 (MQ=255)                                                                           ccGGAGAGGTTGGTTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATa         >  2:315854/1‑90 (MQ=255)                                                                                  ggttggttCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGa  <  1:216116/90‑1 (MQ=255)                                                                                       |                                                                                     GCCCGGCATCTGGATGGCAGAAAAACTGTCAGAATTACCCTCCGCCTGGAGCGTGGCGCATTACGTTGAGTTAACCGGAGAGGTTGATTCGCCATTACTGGCCCGCGCGGTGGTTGCCGGACTAGCGCAAGCAGATACGCTGCGGATGCGTTTTACGGAAGATAACGGCGA  >  NZ_CP009273/609645‑609815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑