Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,119,379	A→G	G44G (GGT→GGC)	grxB ←	glutaredoxin 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,119,379	0	A	G	100.0%	16.5 / NA	7	G44G (GGT→GGC)	grxB	glutaredoxin 2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/3);  total (4/3)

TTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCA  >  NZ_CP009273/1119299‑1119461                                                                                 |                                                                                   ttATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGGCCGACCATc                                                                           <  1:209650/90‑1 (MQ=255)             tGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGGCCGACCATCCGGGGGGGTGtt                                                               >  2:39057/1‑90 (MQ=255)                          tGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGGCCGACCATCCGGGTGGGTGTTTCTGcgtcgtcgt                                                  <  1:439836/90‑1 (MQ=255)                             tttCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGGCCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGa                                               >  2:13320/1‑90 (MQ=255)                               tCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGGCCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGc                                             <  2:180863/90‑1 (MQ=255)                                          gCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGGCCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTaa                                  >  2:215529/1‑90 (MQ=255)                                                                          gTTTTTGGCCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCa  >  1:225238/1‑90 (MQ=255)                                                                                 |                                                                                   TTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCA  >  NZ_CP009273/1119299‑1119461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑