Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,320,565	G→A	Q521Q (CAG→CAA)	yciQ →	DUF2207 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,320,565	0	G	A	100.0%	34.0 / NA	10	Q521Q (CAG→CAA)	yciQ	DUF2207 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/6);  total (4/6)

AGCGGGTTATTTTACCGCTGTTTGCCTGACCGGTTATCTCACTGGCATGGGGTATATTTTTCTGCCAAAGTTTACCCAAACTGGACAGCAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAACACACAGTGGACGTCGGCGGAAA  >  NZ_CP009273/1320478‑1320646                                                                                        |                                                                                  aGCGGGTTATTTTACCGCTGTTTGCCTGACCGGTTATCTCACTGGCATGGGGTATATTTTTCTGCCAAAGTTTACCCAAACTGGacaaca                                                                                 >  2:411372/1‑90 (MQ=255)  gCGGGTTATTTTACCGCTGTTTGCCTGACCGGTTATCTCACTGGCATGGGGTATATTTTTCTGCCAAAGTTTACCCAAACTGGAcaacaa                                                                                >  1:431885/1‑90 (MQ=255)                         ccTGACCGGTTATCTCACTGGCATGGGGTATATTTTTCTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGCTAt                                                         <  1:411372/90‑1 (MQ=255)                          cTGACCGGTTATCTCACAGGCATGGGGTATATTTTTCTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGCTATc                                                        <  1:193946/90‑1 (MQ=255)                                      ctcACTGGCATGGGGTATATTTTTCTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCtt                                            >  2:170613/1‑90 (MQ=255)                                                  gggTATATTTTTCTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAagag                                <  1:110616/90‑1 (MQ=255)                                                      atatTTTTCTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGgcag                            >  1:45885/1‑90 (MQ=255)                                                            ttCTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAacac                      <  2:45885/90‑1 (MQ=255)                                                              cTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGCTa                                                          <  1:155827/52‑1 (MQ=255)                                                              cTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGCTa                                                          >  2:155827/1‑52 (MQ=255)                                                              cTGCCAAAGTTTACCCAAACTGGACAACAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAacacac                    >  2:376211/1‑90 (MQ=255)                                                                                aCTGGACAACAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAACACACAGTGGACGTCGGCGGaaa  <  1:170613/90‑1 (MQ=255)                                                                                        |                                                                                  AGCGGGTTATTTTACCGCTGTTTGCCTGACCGGTTATCTCACTGGCATGGGGTATATTTTTCTGCCAAAGTTTACCCAAACTGGACAGCAACGTTATGCCCACGGTGAAGCTATCGTTAACTATCTTGCGCGTAAAGAGGCAGCAACACACAGTGGACGTCGGCGGAAA  >  NZ_CP009273/1320478‑1320646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑