Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,335,042	A→G	G76G (GGA→GGG)	lapB →	lipopolysaccharide assembly protein LapB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,335,042	0	A	G	100.0%	35.2 / NA	13	G76G (GGA→GGG)	lapB	lipopolysaccharide assembly protein LapB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/7);  total (6/7)

GTAATCAACAGGATAAAGCGGTAGACCTGTTTCTCGATATGCTTAAAGAGGATACAGGCACCGTTGAAGCCCACCTTACGCTCGGAAACCTGTTCCGTTCGCGTGGCGAAGTTGATCGCGCTATTCGCATCCATCAGACCCTAATGGAAAGCGCCTCGCTGACCT  >  NZ_CP009273/1334957‑1335121                                                                                      |                                                                                gTAATCAACAGGATAAAGCGGTAGACCTGTTTCTCGATATGCTTAAAGAGGATACAGGCACCGTTGAAGCCCACCTTACGCTCGGGAAcc                                                                             <  2:315422/90‑1 (MQ=255)                aaGCGGTAGACCTGTTTCTCGATATGCTTAAAGAGGATACAGGCACCGTTGAAGCCCACCTTACGCTCGGGAACCTGTTCCGTTCGCGTg                                                              >  1:445975/1‑90 (MQ=255)                 aGCGGTAGACCTGTTTCTCGATATGCTTAAAGAGGATACAGGCACCGTTGAAGCCCACCTTACGCTCGGGAACCTGTTCCGTTCGCGTgg                                                             <  1:425404/90‑1 (MQ=255)                     gTAGACCTGTTTCTCGATATGCTTAAAGAGGATACAGGCACCGTTGAAGCCCACCTTACGCTCGGGAACCTGTTCCGTTCGCGTGGCGaa                                                         >  2:399616/1‑90 (MQ=255)                                                  ggATACAGGCACCGTTGAAGCCCACCTTACGCTCGGGAACCTGTTCCGTTCGCGTGGCGAAGTTGATCGCGCTATTCGCATCCATCAGAc                            >  1:266130/1‑90 (MQ=255)                                                  ggATACAGGCACCGTTGAAGCCCACCTTACGCTCGGGAACCTGTTCCGTTCGCGTGGCGAAGTTGATCGCGCTATTCGCATCCATCAGAc                            <  1:321126/90‑1 (MQ=255)                                                               gTTGAAGCCCACCTTACGCTCGGGAACCTGTTCCGTTCGCGTGGCGAAGTTGATCGCGCTATTCGCATCCATCAGACCCTAATGGAAAgc               >  2:372077/1‑90 (MQ=255)                                                                 tGAAGCCCACCTTACGCTCGGGAACCTGTTCCGTTCGCGTGGCGAAGTTGATCGCGCTATTCGCATCCATCAGACCCTAATGGAAAgcgc             <  1:372077/90‑1 (MQ=255)                                                                       ccACCTTACGCTCGGGAACCTGTTCCGTTCGCGTGGCGAAGTTGATCGCGCTATTCGCATCCATCAGACCCTAATGGAAAGCGCCTCGCt       >  2:461852/1‑90 (MQ=255)                                                                        cACCTTACGCTCGGGAACCTGTTCCGTTCGCGTGGCGAAGTTGATCGCGCTATTCGCATCCATCAGACCCTAATGGAAAGCGCCTCGCTg      <  2:124974/90‑1 (MQ=255)                                                                        cACCTTACGCTCGGGAACCTGTTCCGTTCGCGTGGCGAAGTTGATCGCGCTATTCGCATCCATCAGACCCTAATGGAAAGCGCCTCGCTg      <  2:460718/90‑1 (MQ=255)                                                                         aCCTTACGCTCGGGAACCTGTTCCGTTCGCGTGGCGAAGTTGATCGCGCTATTCGCATCCATCAGACCCTAATGGAAAGCGCCTCGCTGa     >  2:237475/1‑90 (MQ=255)                                                                            ttACGCTCGGGAACCTGTTCCGTTCGCGTGGCGAAGTTGATCGCGCTATTCGCATCCATCAGACCCTAATGGAAAGCGCCTCGCTGACCt  <  2:199182/90‑1 (MQ=255)                                                                                      |                                                                                GTAATCAACAGGATAAAGCGGTAGACCTGTTTCTCGATATGCTTAAAGAGGATACAGGCACCGTTGAAGCCCACCTTACGCTCGGAAACCTGTTCCGTTCGCGTGGCGAAGTTGATCGCGCTATTCGCATCCATCAGACCCTAATGGAAAGCGCCTCGCTGACCT  >  NZ_CP009273/1334957‑1335121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑