| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 115,628 | A→C | T137P (ACG→CCG) | ampD → | 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase AmpD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 115,628 | 0 | A | C | 88.2% | 30.4 / ‑4.8 | 17 | T137P (ACG→CCG) | ampD | 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase AmpD |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (8/7); minor base T (2/0); total (10/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAAC > NZ_CP009273/115550‑115707 | ttttCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTTCGCGGGCACtg > 1:118750/1‑90 (MQ=255) ttCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACtgat > 1:466608/1‑90 (MQ=255) cTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACtgattg < 2:179005/90‑1 (MQ=255) gggATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGc < 2:296717/90‑1 (MQ=255) gATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGGACTGATTGATTGCTa > 1:52173/1‑90 (MQ=255) gCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGAc < 2:17240/90‑1 (MQ=255) cTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACg < 1:264884/90‑1 (MQ=255) ggCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCcgc > 1:187577/1‑44 (MQ=255) ggCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCcgc < 2:187577/44‑1 (MQ=255) cGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGcc < 2:437998/90‑1 (MQ=255) gTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAAAATGACGGGccc > 2:377871/1‑89 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATc > 1:154282/1‑63 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATc < 2:154282/63‑1 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAAAATGACGGGGCCTTGTGat > 2:120390/1‑90 (MQ=255) tCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTTCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCCGa > 2:87860/1‑90 (MQ=255) aaCAGCTTGCGGCGGTTCCGCGGGCACTGATTTATTGCTATCCCGATATCGCTAAAAACATGACGGGCCATTGTGGTTTTGTGCCGGGTc > 2:416706/1‑90 (MQ=255) tGCGGCGGTTTCGCCGGCACTGATTGATTGCTTTCCGGATATCGCTAAAAAAATGACGGGCCCTTGTGATATTTTTCCGGATCGGAAAAc > 2:115548/1‑90 (MQ=255) | TTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAAC > NZ_CP009273/115550‑115707 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |