Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,454,774	A→G	D16G (GAT→GGT)	paaI →	hydroxyphenylacetyl‑CoA thioesterase PaaI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,454,774	0	A	G	100.0%	69.9 / NA	23	D16G (GAT→GGT)	paaI	hydroxyphenylacetyl‑CoA thioesterase PaaI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/13);  total (10/13)

GCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAA  >  NZ_CP009273/1454699‑1454857                                                                            |                                                                                    gcgcCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAg                                                                       >  1:95838/1‑90 (MQ=255)      aaCGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCtt                                                                  >  1:434520/1‑90 (MQ=255)            cgcTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATc                                                            <  2:95838/90‑1 (MQ=255)                tCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACa                                                        <  2:267139/90‑1 (MQ=255)                     agagCGGCTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTAtc                                                   <  1:99654/90‑1 (MQ=255)                        gCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCa                                                >  2:139456/1‑90 (MQ=255)                        gCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCa                                                >  1:325808/1‑90 (MQ=255)                          ggTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAAt                                              <  1:339530/90‑1 (MQ=255)                          ggTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATAGACATTATCTCAAt                                              >  2:375291/1‑90 (MQ=255)                                    cATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGc                                    <  1:159189/90‑1 (MQ=255)                                    cATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGc                                    <  1:116413/90‑1 (MQ=255)                                    cATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGc                                    <  2:251164/90‑1 (MQ=255)                                     aTAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGc                                    >  2:180876/1‑89 (MQ=255)                                     aTAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGc                                    <  1:180876/89‑1 (MQ=255)                                     aTAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCt                                   >  2:424183/1‑90 (MQ=255)                                     aTAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGAGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCt                                   >  2:288832/1‑90 (MQ=255)                                      tAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCtt                                  <  1:186144/90‑1 (MQ=255)                                      tAAGGCCTGGCAAAACGCCCATGGAATGTATGAGATCGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCtt                                  <  2:241531/90‑1 (MQ=255)                                            cTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGt                            >  1:384231/1‑90 (MQ=255)                                                                     gagaACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACaa   >  1:29388/1‑90 (MQ=255)                                                                      agaACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACaaa  <  2:438954/90‑1 (MQ=255)                                                                      agaACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACaaa  <  2:6584/90‑1 (MQ=255)                                                                      aaaaCGGTGCCCGCGCCACTGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTAACCATGACAGTGACTGCACaac  <  2:243809/88‑2 (MQ=255)                                                                            |                                                                                    GCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAA  >  NZ_CP009273/1454699‑1454857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑