Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,499,566	A→G	S258P (TCC→CCC)	ydcO ←	BenE family transporter YdcO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,499,566	0	A	G	100.0%	30.6 / NA	11	S258P (TCC→CCC)	ydcO	BenE family transporter YdcO
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/5);  total (6/5)

GTTGATCTTTATCCGGATGCGCTTCCGGGCTTTGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGAAAAAACCAGTGCCAGCAATCCAGTAAATACAATTAATGGCGAAACAGGAGCCGAATATCCAGCTGCTTTCATTGCTGCGA  >  NZ_CP009273/1499479‑1499646                                                                                        |                                                                                 gTTGATCTTTATCCGGATGCGCTTCCGGGCTTTGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGgaa                                                                                <  2:4924/90‑1 (MQ=255)         ttATCCGGATGCGCTTCCGGGCTTTGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGGAAAAACCAGt                                                                        >  2:340879/1‑90 (MQ=255)                   gcgcTTCCGGGCTTTGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGGAAAAACCAGTGCCAGCAATc                                                              <  2:29505/90‑1 (MQ=255)                                ttGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGGAAAAACCAGTGCCAGCAATCCAGTAAATACAAt                                                 >  1:213731/1‑90 (MQ=255)                                     aaaTAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGGAAAAACCAGTGCCAGCAATCCAGTAAATACAATTAATg                                            >  1:457661/1‑90 (MQ=255)                                               ggTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGGAAAAACCAGTGCCAGCAATCCAGTAAATACAATTAATGGCGAAACAgg                                  <  2:21869/90‑1 (MQ=255)                                                         gCAATACCGACGGAATAAACGCCGAAAGGGGGAAAAACCAGTGCCAGCAATCCAGTAAATACAATTAATGGCGAAACAGGAGCCGAatat                        <  1:93250/90‑1 (MQ=255)                                                             tACCGACGGAATAAACGCCGAAAGGGGGAAAAACCAGTGCCAGCAATCCAGTAAATACAATTAATGGCGAAACAGGAGCCGAATATCCAg                    <  2:461465/90‑1 (MQ=255)                                                                 gACGGAATAAACGCCGAAAGGGGGAAAAACCAGTGCCAGCAATCCAGTAAATACAATTGATGGCGAAACAGGAGCCGAATATCCAgctgc                >  2:150727/1‑90 (MQ=255)                                                                          aaCGCCGAAAGGGGGAAAAACCAGTGCCAGCAATCCAGTAAATACAATTAATGGCGAAACAGGAGCCGAATATCCAGCTGCTTTCATtgc       <  1:393746/90‑1 (MQ=255)                                                                               cGAAAGGGGGAAAAACCAGTGCCAGCAATCCAGTGAATACAATTAATGGCGAAACAGGAGCCGAATATCCAGCTGCTTTCATTGCTGCGa  >  1:321474/1‑90 (MQ=255)                                                                               cGAAAGGGGGAAAAACCAGTGCCAGCAATCCAGTAAATACAATTAATGGCGAAACAGGAGCCGAATATCCAGCTGCTTTCATTGCTGCGa  >  1:451812/1‑90 (MQ=255)                                                                                        |                                                                                 GTTGATCTTTATCCGGATGCGCTTCCGGGCTTTGGCAAATAGCCGCGGTGATTGCCGCAATACCGACGGAATAAACGCCGAAAGGGGAAAAAACCAGTGCCAGCAATCCAGTAAATACAATTAATGGCGAAACAGGAGCCGAATATCCAGCTGCTTTCATTGCTGCGA  >  NZ_CP009273/1499479‑1499646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑