Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,616,259	C→T	A224V (GCA→GTA)	ydeE →	efflux MFS transporter YdeE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,616,259	0	C	T	100.0%	44.1 / NA	13	A224V (GCA→GTA)	ydeE	efflux MFS transporter YdeE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/4);  total (9/4)

GGTCGCCGAAAGTTTTATTACAAGATAAAGCACTGTTGTGGTTTACCTGCTCTGGTTTTCTGGCTTCTTTTGTAAGCGGCGCATTTGCTTCATGCATTTCACAATATGTGATGGTGATTGCTGATGGGGATTTTGCCGAAAAGGTGGTCGCGGTTG  >  NZ_CP009273/1616178‑1616333                                                                                  |                                                                           ggTCGCCGAAAGTTTTATTACAAGATAAAGCACTGTTGTGGTTTACCTGCTCTGGTTTTCTGGCTTCTTTTGTAAGCGGCGTATTTGCtt                                                                    >  1:205069/1‑90 (MQ=255)             ttttATTACAAGATAAAGCACTGTTGTGGTTTACCTGCTCTGGTTTTCTGGCTTCTTTTGTAAGCGGCGTATTTGCTTCATGCATTTcac                                                        <  2:403152/90‑1 (MQ=255)                            aaGCACTGTTGTGGTTTACCTGCTCTGGTTTTCTGGCTTCTTTTGTAAGCGGCGTATTTGCTTCATGCATTTCACAATATGTGATGGTGa                                         >  1:232153/1‑90 (MQ=255)                                             aCCTGCTCTGGTTTTCTGGCTTCTTTTGTAAGCGGCGTATTTGCTTCATGCATTTCACAATATGTGATGGTGATTGCTGATGGGGAtttt                        >  1:147865/1‑90 (MQ=255)                                             aCCTGCTCTGGTTTTCTGGCTTCTTTTGTAAGCGGCGTATTTGCTTCATGCATTTCACAATATGTGATGGTGATTGCTGATGGGGAtttt                        >  1:149019/1‑90 (MQ=255)                                                 gCTCTGGTTTTCTGGCTTCTTTTGTAAGCGGCGTATTTGCTTCATGCATTTCACAATATGTGATGGTGATTGCTGATGGGGATTTTGCCg                    >  1:3434/1‑90 (MQ=255)                                                  ctctGGTTTTCTGGCTTCTTTTGTAAGCGGCGTATTTGCTTCATGCATTTCACaa                                                      >  1:84502/1‑55 (MQ=255)                                                  ctctGGTTTTCTGGCTTCTTTTGTAAGCGGCGTATTTGCTTCATGCATTTCACaa                                                      <  2:84502/55‑1 (MQ=255)                                                  ctctGGTTTTCTGGCTTCTTTTGTAAGCGGCGTATTTGCTTCATGCATTTCACAATATGTGATGGTGATTGCTGATGGGGATTTTGCCGa                   >  1:98310/1‑90 (MQ=255)                                                       gTTTTCTGGCTTCTTTTGTAAGCGGCGTATTTGCTTCATGCATTTCACAATATGTGATGGTGATTGCTGATGGGGATTTTGCCGAAAAgg              <  1:357217/90‑1 (MQ=255)                                                           tCTGGCTTCTTTTGTAAGCGGCGTATTTGCTTCATGCATTTCACAATATGTGATGGTGATTGCTGATGGGGATTTTGCCGAAAAggtggt          >  1:78801/1‑90 (MQ=255)                                                                  tcttTTGTAAGCGGCGTATTTGCTTCATGCATTTCACAATATGTGATGGTGATTGCTGATGGGGATTTTGCCGAAAAGGTGGTCGCGgtt   <  2:98310/90‑1 (MQ=255)                                                                   cttTTGTAAGCGGCGTATTTGCTTCATGCATTTCACAATATGTGATGGTGATTGCTGATGGGGATTTTGCCGAAAAGGTGGTCGCGgttg  >  1:54105/1‑90 (MQ=255)                                                                                  |                                                                           GGTCGCCGAAAGTTTTATTACAAGATAAAGCACTGTTGTGGTTTACCTGCTCTGGTTTTCTGGCTTCTTTTGTAAGCGGCGCATTTGCTTCATGCATTTCACAATATGTGATGGTGATTGCTGATGGGGATTTTGCCGAAAAGGTGGTCGCGGTTG  >  NZ_CP009273/1616178‑1616333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑