| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 340,444 | (A)5→4 | coding (558/816 nt) | BW25113_RS01680 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 340,440 | 0 | A | . | 96.5% | 110.7 / ‑1.8 | 29 | coding (554/816 nt) | BW25113_RS01680 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base . (18/10); total (19/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01 | |||||||||||
GTGCGAACAGATTGCAAATGTATTCTTCTTGAATACCATATGCCTTTGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGGAAAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCATGGATTATTATTACACTTA > NZ_CP009273/340352‑340525 | gTGCGAACAGATTGCAAATGTATTCTTCTTGAATACCATATGCCTTTGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGGaa > 1:302373/1‑90 (MQ=255) aTTGCAAATGTATTCTTCTTGAATACCATATGCCTTTGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTcg > 1:238550/1‑90 (MQ=255) aTTGCAAATGTATTCTTCTTGAATACCATATGCCTTTGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTcg > 1:405686/1‑90 (MQ=255) aTTGCAAATGTATTCTTCTTGAATACCATATGCCTTTGCTGAGAATTTTCCCTAAAGGG‑AAGAGTGTATGCATGTGG‑AAAATGGCTTcgc > 2:107704/1‑90 (MQ=255) ttcttcTTGAATACCATATGCCTTTGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATgtcgtc < 1:226576/90‑1 (MQ=255) tcttGAATACCATATGCCTTTGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTAtt > 1:191748/1‑90 (MQ=255) tcttGAATACCATATGCCTTTGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTAtt > 1:2420/1‑90 (MQ=255) atatGCCTTTGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGagacaga > 2:93097/1‑90 (MQ=255) tGCCTTTGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGAcgc > 2:324885/1‑90 (MQ=255) cccTTTGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGAcgcg < 2:9086/89‑1 (MQ=255) ttGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCa > 1:387880/1‑90 (MQ=255) ttGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCa > 1:332846/1‑90 (MQ=255) ttGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCa > 2:360756/1‑90 (MQ=255) ttGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCa > 1:504416/1‑90 (MQ=255) ttGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCa > 1:53083/1‑90 (MQ=255) aaGGG‑AAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCAt < 1:107704/90‑1 (MQ=255) aGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCAt < 2:238550/90‑1 (MQ=255) aGGGAAAGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCAt < 2:423303/90‑1 (MQ=255) aaGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCATGGatt < 1:84364/90‑1 (MQ=255) aaGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCATGGatt < 2:332846/90‑1 (MQ=255) aaGAGTGTATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCATGGatt < 2:504416/90‑1 (MQ=255) gtgtATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCtt < 1:487933/75‑1 (MQ=255) gtgtATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCtt > 2:487933/1‑75 (MQ=255) gtgtATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCATGGattatta > 2:289012/1‑90 (MQ=255) gtATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGAcgc < 1:70858/54‑1 (MQ=255) gtATGCATGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGAcgc > 2:70858/1‑54 (MQ=255) aTGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCATGGATTATTATTACACtt > 1:252244/1‑90 (MQ=255) aTGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCATGGATTATTATTACACtt > 1:467710/1‑90 (MQ=255) aTGTGG‑AAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCATGGATTATTATTACACtt > 1:42211/1‑90 (MQ=255) gtgGAAAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCATGGATTATTATTACACTTa > 1:295792/1‑90 (MQ=255) | GTGCGAACAGATTGCAAATGTATTCTTCTTGAATACCATATGCCTTTGCTGAGAATTTTCCCTAAAGGGAAAGAGTGTATGCATGTGGAAAAATGGCTTCGCGAAATGTCGTCTATTCCGGAGACAGACGCGCCCATTAAGCAGGCGCTTGCGCATGGATTATTATTACACTTA > NZ_CP009273/340352‑340525 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |