| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 754,027 | 0 | T | A | 53.8% | 3.8 / 16.7 | 13 | intergenic (+166/‑135) | sdhB/sucA | succinate dehydrogenase iron‑sulfur subunit SdhB/2‑oxoglutarate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base T (5/1); new base A (0/7); total (5/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAA‑CTG‑CCCCTG‑ACACTAAGACAGTTTTTAAAGG‑TT‑CCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTA > NZ_CP009273/753945‑754115 | tCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAA‑CTG‑CCCCTG‑ACACTAAGACAg < 2:369523/90‑1 (MQ=255) ttACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAA‑CTG‑CCCCTG‑ACACTAAGACAGttt > 2:150655/1‑90 (MQ=255) gTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAA‑CTG‑CCCCTG‑ACACTAAGACAGTTTTTAAAg > 1:366117/1‑90 (MQ=255) tAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAA‑CTG‑CCCCTG‑ACACTAAGACAGTTTTTAAAgg > 1:119298/1‑90 (MQ=255) ggcgcaGCGGGCTAAAAAAAAAAATGAAAAAAAT‑TTTAAAAA‑CCG‑CCCCCGCCCACAAAGACA‑TTTTTAAAGGTTT‑CCTTCGCGAGCCAc < 2:212045/89‑1 (MQ=255) tAACAAAGAAATGCAGGAAATCTTTAAAAA‑CTG‑CCCCTG‑ACACTAAGACAGTTTTTAAAGG‑TT‑CCTTCGCGAGCCACTACGTAGACAaga > 1:87538/1‑90 (MQ=255) tttAAAAACCTC‑CCCCTG‑ACACAAAGACATTTTTTAAAGG‑TT‑CCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGgca < 1:430423/90‑1 (MQ=255) tttAAAAA‑CCCCCCCCTG‑ACACAAAGACATTTTTAAAAGT‑TC‑CCTCCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGgca < 2:87538/90‑1 (MQ=255) tttAAAAA‑CCCCCCCCTG‑ACAAAAAGACA‑TTTTTAAAGG‑TTCCCTCCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGgca < 2:416963/90‑1 (MQ=255) aaaac‑CTC‑CCCCTG‑ACACAAAGACATTTTTTAAAGG‑TT‑CCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGgcacgca < 1:12949/90‑1 (MQ=255) ccccTA‑ACACAAAGACATTTTTTAAGGG‑TC‑CCTCCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACtg < 2:104882/90‑1 (MQ=255) ccTG‑ACAAAAAGACTTTTTTAAAAGT‑TC‑CCTCCGGGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACtgtg < 2:366117/90‑1 (MQ=255) cTAAGACAGTTTTTAAAGG‑TT‑CCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGgta > 2:254759/1‑90 (MQ=255) | TCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAA‑CTG‑CCCCTG‑ACACTAAGACAGTTTTTAAAGG‑TT‑CCTTCGCGAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCACATCACTGTGCGTGGTA > NZ_CP009273/753945‑754115 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |