Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,385,3480TC54.5% 3.0 / 11.5 11S255G (AGC→GGC) ycjYalpha/beta hydrolase
Reads supporting (aligned to +/- strand):  ref base T (5/0);  new base C (0/6);  total (5/6)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TTGCGCCTTCAACAATGTGATAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCTGCCTGGCTGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATC  >  NZ_CP009273/1385273‑1385430
                                                                           |                                                                                  
ttGCGCCTTCAACAATGTGATAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCTGCCTGGCTGc                                                                      >  1:428111/1‑90 (MQ=255)
                      gcttttACCCTGGCTTGATGCTCGATCGTACGGGTCGTCCCTCATCCTTTTGCCCCCTGCCTGGCTGCCCGCCACAATCTGCGTCGGCTg                                                <  1:265365/87‑1 (MQ=255)
                        gTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCTGCCTGGCTGCCCGCCACAATCTGCGTCGGCTGAg                                              >  1:43682/1‑90 (MQ=255)
                         tttACCCTGGTTTGATGCTCGATCGTACGGGTCGTCACTCATCCTTTTGCCCCCTGCCTGGCTGCCCGCCACAATCTGCGTCGGCTGAGt                                             <  2:37144/90‑1 (MQ=255)
                          ttATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCTGCCTGGCTGCCCGCCACAATCTGCGTCGGCTGAGTc                                            >  1:12293/1‑90 (MQ=255)
                          ttATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCTGCCTGGCTGCCCGCCACAATCTGCGTCGGCTGAGTc                                            >  1:72714/1‑90 (MQ=255)
                                      gATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCTGCCTGGCTGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTcc                                >  2:162059/1‑90 (MQ=255)
                                          cTCGATCGTACGGGTCGTCACTCATCCTTTTGCCCCCTGCCTGGCTCCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCa                            <  1:64310/90‑1 (MQ=255)
                                                 gTACGGTTCGTCCCTCACCTTTTTCCCCCCTGCCGGCCTGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTa                     <  2:428111/90‑1 (MQ=255)
                                                   aCAGGTCGCCACTCACCTTTTTCCCCCCTCCCGGCCTCCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAg                   <  2:72714/90‑1 (MQ=255)
                                                                    cttttGCCCCCTCCCTGGCTCCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATc  <  2:43682/88‑1 (MQ=255)
                                                                           |                                                                                  
TTGCGCCTTCAACAATGTGATAGCGTTTATCCTGGCTTGATGCTCGATCGTACAGGTCGTCACTCATCCATTTGCTCCCTGCCTGGCTGCCCGCCACAATCTGCGTCGGCTGAGTCAGGTACACTTCCGCCATATGGTAAGCATCATAGGTAATAATC  >  NZ_CP009273/1385273‑1385430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑