| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,033,556 | 0 | C | G | 52.9% | 13.0 / 24.6 | 17 | L200V (CTG→GTG) | msrP | protein‑methionine‑sulfoxide reductase catalytic subunit MsrP |
| Reads supporting (aligned to +/- strand): ref base C (0/8); new base G (9/0); total (9/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.10e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGCAA > NZ_CP009273/2033473‑2033638 | gCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAat < 2:256635/90‑1 (MQ=255) aaTGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGGGGGGTGGAaaat > 1:398481/1‑90 (MQ=255) aTGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGGGGGGTGGAaaata > 1:375712/1‑89 (MQ=255) aTGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGGGGGGTGGAAAATc > 1:232174/1‑90 (MQ=255) aaGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGGGGGGTGAAAAAACCtttt > 2:1772/1‑88 (MQ=255) tGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGGGGGGGGAAAAAATCTTATTTCgg > 1:294247/1‑89 (MQ=255) ttCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGGGGAAAATCCTTATGTTGAAGGAATg > 1:484800/1‑90 (MQ=255) ttCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGGGGGGTGAAAAATCCTTATGTCGAAGGATTg > 1:139630/1‑90 (MQ=255) aTTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTcgac < 1:74050/90‑1 (MQ=255) cGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGGAATATTCTTATTTCGAAGGAATGCGTCTTGACGAAGCAATga > 1:348295/1‑89 (MQ=255) gAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCAt < 2:219772/90‑1 (MQ=255) tGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTca < 1:282604/90‑1 (MQ=255) gccagCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACtga < 2:398481/90‑1 (MQ=255) ccGCTTTATCGGCGGTGGGCTGAAATGTCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAgg < 1:344482/90‑1 (MQ=255) ccGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAgg < 2:60123/90‑1 (MQ=255) ccGCTTTATCGGCGGGGGGGTGGAAAATCCTTTTGTCGAAGGAGTGCGTCTCGACGAAGGAATGCATCCGCCCACACTGATGAACGGAgg > 2:206349/1‑90 (MQ=255) cggTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGCaa < 1:189806/90‑1 (MQ=255) | GCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGCAA > NZ_CP009273/2033473‑2033638 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |