| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,786,319 | 0 | A | C | 53.3% | 9.2 / 14.8 | 15 | T76P (ACC→CCC) | gabT | 4‑aminobutyrate‑‑2‑oxoglutarate transaminase |
| Reads supporting (aligned to +/- strand): ref base A (3/4); new base C (0/8); total (3/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.69e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.22e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGGGCGGGATTGCGGTGCTCAATACCGGGCACCTGCATCCGAAGGTGGTGGCCGCGGTGGAAGCGCAGTTGAAAAAACTGTCGCACACCTGCTTCCAGGTGCTGGCTTACGAGCCGTATCTGGAGCTGTGCGAGATTATGAATCAGAAGGTGCCGGGCGATT > NZ_CP009273/2786232‑2786394 | gcgggcggGATTGCGGTGCTCAATACCGGGCACCTGCATCCGAAGGTGGTGGCCGCGGTGGAAGCGCAGTTGAAAAAACTGTCGCACAcc < 2:11705/90‑1 (MQ=255) cGGTGCTCAATACCGGGCACCTGCATCCGAAGGTGGTGGCCGCGGTGGAAGCGCAGTTGAAAAAACTGTCGCACACCTGCTTCCAGGTGc < 2:241962/90‑1 (MQ=255) aTACCGGGCCCCTGCACCCGAGGGTGGGGGCCGGGGTGGAAGCGCTGTTAAAAAACCTGTCGCCCACCTGCTTCCAGGTGCTGGTTTACg < 2:327967/90‑1 (MQ=255) gggCCCCTGCACCCGAAGGTGGTGGCCGCGGTGGAAGCGCAGTTGAAAAAACTGTCGCACCCCTGCTTCCAGGTGCTGGTTTACGAGCCg < 1:355547/90‑1 (MQ=255) gggCACCTGCATCCGAGGGTGGGGGCCGCGGTGGAAGCGCAGTTAAAAAAACTGTCGCACCCCTGCTTCCAGGTGCTGGTTTACGAGCCg < 2:347415/90‑1 (MQ=255) gggCACCTGCATCCGAGGGGGGTGGCCGGGGTGGAAGCGCAGTTGAAAAAACTGTCGCACCCCTGCTTCCAGGTGCTGGTTTAGGAGCCg < 2:335010/90‑1 (MQ=255) gggCACCTGCATCCGAAGGTGGTGGCCGCGGTGGAAGCGCAGTTGAAAAAACTGTCGCACCCCTGCTTCCAGGTGCTGGCTTACGAGCCg < 1:216769/90‑1 (MQ=255) gggCACCTGCATCCGAAGGTGGTGGCCGCGGTGGAAGCGCAGTTGAAAAAACTGTCGCACACCTGCTTCCAGGTGCTGGCTTACGAGCCg > 1:347415/1‑90 (MQ=255) gggCACCTGCATCCGAAGGTGGTGGCCGCGGTGGAAGCGCAGTTGAAAAAACTGTCGCACACCTGCTTCCAGGTGCTGGCTTACGAGCCg > 2:152478/1‑90 (MQ=255) aTCCGAAGGTGGTGGCCGCGGTGGAAGCGCAGTTGAAAAAACTGTCGCACCCCTGCTTCCAGGTGCTGGCTTACGAGCCGTATCTGGAGc < 1:71878/90‑1 (MQ=255) gtggtgGCCGCGGGGGAAGCGCAGTTGAAAAACCTGTCGCACCCCTGCTTCCGGGTGCTGGTTTCCGAGCCGTATCTGGAGCTGTGCgag < 2:101540/90‑1 (MQ=255) ggggtggCCGGGGGGGGAGAGGTGTTGAAAAACCTGCCGCTCCCGTGCTCACAGGTGCGGGTTGACGAGCCGTATCTGGAGCTGTGCgag < 2:480179/88‑1 (MQ=255) ggtggCCGCGGTGGAAGCGCAGTTGAAAAAACTGTCGCACACCTGCTTCCAGGTGCTGGCTTACGAGCCGTATCTGGAGCTGTGCGAGAt > 1:88359/1‑90 (MQ=255) gCCGCGGTGGAAGCGCAGTTGAAAAAACTGTCGCACACCTGCTTCCAGGTGCTGGCTTACGAGCCGTATCTGGAGCTGTGCGAGATTATg < 1:152478/90‑1 (MQ=255) aaaaaCTGTCCCCCCCCTGCTTCCAGGTGCTGGCTTACGAGCCGTATCTGGAGCTGTGCGAGATTATGAATCAGAAGGTGCCGGGCGAtt < 1:472816/90‑1 (MQ=255) | GCGGGCGGGATTGCGGTGCTCAATACCGGGCACCTGCATCCGAAGGTGGTGGCCGCGGTGGAAGCGCAGTTGAAAAAACTGTCGCACACCTGCTTCCAGGTGCTGGCTTACGAGCCGTATCTGGAGCTGTGCGAGATTATGAATCAGAAGGTGCCGGGCGATT > NZ_CP009273/2786232‑2786394 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |