| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,213,521 | 0 | T | G | 53.8% | 8.7 / 12.5 | 13 | K12N (AAA→AAC) | iclR | glyoxylate bypass operon transcriptional repressor IclR |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (7/0); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.59e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCT > NZ_CP009273/4213439‑4213609 | aTTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGgttttttc > 2:431607/1‑86 (MQ=255) tCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCcg > 1:521172/1‑90 (MQ=255) cGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGcccc > 1:516468/1‑88 (MQ=255) ccAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGaa < 1:383074/90‑1 (MQ=255) gTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGGTTCGCGGGGATgg > 1:305284/1‑90 (MQ=255) ttCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTGCCGCGGTTCGCGGGAATGGGGGCg > 1:96135/1‑90 (MQ=255) gAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTTTGCCGCGGTTCGCGGGGATGGGGGCGACCATGACAGTCTCtttttttt > 1:190213/1‑88 (MQ=255) gAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGGTTCGCGGGGATGGGGGGGGCCCTGAAAGTCTCtttttttt > 1:271574/1‑88 (MQ=255) cTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTAt < 2:240567/90‑1 (MQ=255) gCTGGTGCGGTGGCAACGGCGGGGTTTTTTCCGCGTTTCGCGGGGAAGGGGGCGGCCATGACAGTCTCTTTTTTTTTTATTGTGGgaaac > 1:240567/1‑88 (MQ=255) tGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGTTTTGCGGGAATGGGGGGGGCCCTGACAGTCTCTTTTTTTTTTTTCGTGGGAAacat > 1:315033/1‑90 (MQ=255) gTTTTCTGCCGCGTTTCGCGGGCCTGGCTGCGACCATGACAGTCACCTTTTCCTGTACCGTGGAAATCATTTTCATTTTTATTGTTAGCt < 1:232351/90‑1 (MQ=255) gTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCt < 1:202390/90‑1 (MQ=255) gTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCt < 2:190213/90‑1 (MQ=255) | ATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCT > NZ_CP009273/4213439‑4213609 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |