Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,736	A→T	intergenic (+105/‑87)	kefC → / → folA	glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,736	0	A	T	100.0%	91.8 / NA	25	intergenic (+105/‑87)	kefC/folA	glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (12/13);  total (12/13)

CTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTT  >  NZ_CP009273/49661‑49806                                                                            |                                                                       cTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAAcatc                                                          <  1:276484/90‑1 (MQ=255) cTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAAcatc                                                          <  2:393966/90‑1 (MQ=255) cTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAAcatc                                                          <  1:522502/90‑1 (MQ=255)  tAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAAcatca                                                         <  2:51970/90‑1 (MQ=255)  tAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAAcatca                                                         <  2:267274/90‑1 (MQ=255)   aaCTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAAcatcat                                                        >  1:523557/1‑90 (MQ=255)     cTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATcc                                                      <  2:298165/90‑1 (MQ=255)         ttgtttttgtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGc                                                  >  2:511689/1‑90 (MQ=255)           gtttttgtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCAc                                                <  1:400059/90‑1 (MQ=255)               ttgtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGt                                            <  2:163150/90‑1 (MQ=255)                            aTGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTa                               <  2:76462/90‑1 (MQ=255)                                ggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCt                           <  1:3835/90‑1 (MQ=255)                                 gcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCtt                          >  1:403548/1‑90 (MQ=255)                                   gAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCtt                          >  2:309457/1‑88 (MQ=255)                                   gAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCtt                          <  1:309457/88‑1 (MQ=255)                                          gggAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTa                        >  2:388125/1‑83 (MQ=255)                                          gggAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTa                        <  1:388125/83‑1 (MQ=255)                                              gagagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGc             >  1:232261/1‑90 (MQ=255)                                               agagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCg            >  1:149843/1‑90 (MQ=255)                                               agagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCg            >  1:303463/1‑90 (MQ=255)                                               agagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCg            >  1:251394/1‑90 (MQ=255)                                                gagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGa           >  1:211705/1‑90 (MQ=255)                                                         cTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAAtttttt  >  1:45714/1‑90 (MQ=255)                                                           cGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCt                           <  2:101237/63‑1 (MQ=255)                                                           cGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCt                           >  1:101237/1‑63 (MQ=255)                                                                            |                                                                       CTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTT  >  NZ_CP009273/49661‑49806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑