Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,875,309	G→C	A253G (GCC→GGC)	dmlR ←	DNA‑binding transcriptional regulator DmlR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,875,309	0	G	C	100.0%	28.4 / NA	10	A253G (GCC→GGC)	dmlR	DNA‑binding transcriptional regulator DmlR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (3/7);  total (3/7)

CTGACGTCGCCAGCCTTGAAACATAAACGGCCCAGACGTTCGCTGGCTGGTAATATTCCGGTAGCACTTGCACTAAATGACCACTGGCAATGTTTTCGCTAACATCCCACCAGGAACGCAGAGCAATCCCCTGCCCATCGAGACACCACTGATGGACAATTTCTCC  >  NZ_CP009273/1875223‑1875388                                                                                       |                                                                                cTGACGTCGCCAGCCTTGAAACATAAACGGCCCAGACGTTCGCTGGCTGGTAATATTCCGGTAGCACTTGCACTAAATGACCACTGCCaa                                                                              <  1:355251/90‑1 (MQ=255)            aGCCTTGAAACATAAACGGCCCAGACGTTCGCTGGCTGGTAATATTCCGGTAGCACTTGCACTAAATGACCACTGCCAATGTTTTCGCTa                                                                   <  2:71060/90‑1 (MQ=255)                         aaaCGGCCCAGACGTTCGCTGGCTGGTAATATTCCGGTAGCACTTGCACTAAATGACCACTGCCAATGTTTTCGCTAACATCCCACCAgg                                                      <  2:408834/90‑1 (MQ=255)                                ccAGACGTTCGCTGGCTGGTAATATTCCGGTAGCACTTGCACTAAATGACCACTGCCAATGTTTTCGCTAACATCCCACCAGGAACGCag                                               <  1:465098/90‑1 (MQ=255)                                        tCGCTGGCTGGTAATATTCCGGTAGCACTTGCACTAAATGACCACTGCCAATGTTTTCGCTAACATCCCACCAGGAACGCAGAGCAATcc                                       >  1:323996/1‑90 (MQ=255)                                             ggctggTAATATTCCGGTAGCACTTGCACTAAATGACCACTGCCAATGTTTTCGCTAACATCCCACCAGGAACGCAGAGCAATCCCCTGc                                  >  1:247812/1‑90 (MQ=255)                                                                    ttGCACTAAATGACCACTGCCAATGTTTTCGCTAACATCCCACCAGGAACGCAGAGCAATCCCCTGCCCATCGAGACACCACTGATGGAc           <  2:247812/90‑1 (MQ=255)                                                                             aTGACCACTGCCAATGTTTTCGCTAACATCCCACCAGGAACGCAGAGCAATCCCCTGCCCATCGAGACACCACTGATGGACAATTTCTcc  <  2:367418/90‑1 (MQ=255)                                                                                   aCTGCCAATGTTTTCGCTAACATCCCACCAGGAACGCAGAGCAATCCCCTGccc                                <  1:264833/54‑1 (MQ=255)                                                                                   aCTGCCAATGTTTTCGCTAACATCCCACCAGGAACGCAGAGCAATCCCCTGccc                                >  2:264833/1‑54 (MQ=255)                                                                                       |                                                                                CTGACGTCGCCAGCCTTGAAACATAAACGGCCCAGACGTTCGCTGGCTGGTAATATTCCGGTAGCACTTGCACTAAATGACCACTGGCAATGTTTTCGCTAACATCCCACCAGGAACGCAGAGCAATCCCCTGCCCATCGAGACACCACTGATGGACAATTTCTCC  >  NZ_CP009273/1875223‑1875388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑