| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,907,386 | T→C | D563G (GAT→GGT) | prc ← | carboxy terminal‑processing peptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,907,386 | 0 | T | C | 88.2% | 38.2 / ‑0.7 | 17 | D563G (GAT→GGT) | prc | carboxy terminal‑processing peptidase |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (6/9); total (7/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGTTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCT > NZ_CP009273/1907306‑1907452 | tCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGTTAAACCTCCTGAtt < 2:54607/90‑1 (MQ=255) atgatgTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGTTAAACCTCCTGATTTc < 2:242871/90‑1 (MQ=255) tgatgTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGTTAAACCTCCTGATTTca > 2:173886/1‑90 (MQ=255) gTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGTTAAACCTCCTGATTTCACATa > 2:523707/1‑90 (MQ=255) ttCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGTTAAACCTCCTGATTTCACATaa > 1:224221/1‑90 (MQ=255) ttCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGTTAAACCTCCTGATTTCACATaa > 2:296877/1‑90 (MQ=255) aaCTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGTTAAACCTCCTGATTTCACATAAGTcgcg < 1:223361/90‑1 (MQ=255) tCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGTTAAACCTCCTGATTTCACATAAGTCGCGGCa > 2:199537/1‑90 (MQ=255) aGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGTTAAACCTCCTGATTTCACATAAGTCGCGGCATc < 2:224221/90‑1 (MQ=255) aGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGTTAAACCTCCTGATTTCACATAAGTCGCGGCATc < 2:492783/90‑1 (MQ=255) aGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGTTAAACCTCCTGATTTCACATAAGTCGCGGCATc < 2:153921/90‑1 (MQ=255) tttCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGTTAAACCTCCTGATTTCACATAAGTCGCGGCATCAATGCt < 2:459705/90‑1 (MQ=255) gcgcATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGTTAAACCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCa < 2:67882/90‑1 (MQ=255) tCAGCAGCTCCGGTTCAAAGGCCGTTAAACCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGa < 2:223023/90‑1 (MQ=255) gcTCCGGTTCAAAGGCCGTTAAACCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTtct > 1:321555/1‑90 (MQ=255) cGTTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCt < 2:376950/42‑1 (MQ=255) cGTTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCt > 1:376950/1‑42 (MQ=255) | TCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGTTAAATCTCCTGATTTCACATAAGTCGCGGCATCAATGCTATCCCACGGCAGCGCGTTATCTTCGAATTTCT > NZ_CP009273/1907306‑1907452 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |