Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,980,420	A→G	G5G (GGA→GGG)	ftnB →	non‑heme ferritin‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,980,420	0	A	G	96.0%	78.2 / ‑3.4	25	G5G (GGA→GGG)	ftnB	non‑heme ferritin‑like protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (13/11);  minor base T (0/1);  total (13/12)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.69e-01

CTGCAAACAGTAATTATGGTGTTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTG  >  NZ_CP009273/1980336‑1980502                                                                                     |                                                                                   cTGCAAACAGTAATTATGGTGTTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCt                                                                               <  2:17517/90‑1 (MQ=255)           tAATTATGGTGTTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAaactc                                                                     <  1:366244/90‑1 (MQ=255)            aaTTATGGTGTTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAaactca                                                                    <  1:386788/90‑1 (MQ=255)                  ggTGTTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACtctc                                                              >  1:327372/1‑90 (MQ=255)                              tATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGTATGCTTCTCAAACTCAACTCTCAAATGAACcgcg                                                  <  2:445190/90‑1 (MQ=255)                                         tctcCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCa                                       >  1:206525/1‑90 (MQ=255)                                         tctcCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCa                                       >  2:189273/1‑90 (MQ=255)                                            ccACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATcc                                    <  2:327372/90‑1 (MQ=255)                                                  cTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAAtctc                              <  2:337026/90‑1 (MQ=255)                                                  cTGGATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAAtctc                              <  2:220738/90‑1 (MQ=255)                                                    ggATATAAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTa                            <  1:389489/90‑1 (MQ=255)                                                         taAGGATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTc                       >  1:350880/1‑90 (MQ=255)                                                            ggATATTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCAcc                    <  2:131009/90‑1 (MQ=255)                                                              atatTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTg                  >  1:159062/1‑90 (MQ=255)                                                              atatTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTg                  >  1:121559/1‑90 (MQ=255)                                                                atTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAg                >  2:166874/1‑90 (MQ=255)                                                                atTAGGTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAg                >  1:397699/1‑90 (MQ=255)                                                                     gTATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTAACt           >  2:317142/1‑90 (MQ=255)                                                                      tATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAAtctc                              >  1:58428/1‑70 (MQ=255)                                                                      tATGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAAtctc                              <  2:58428/70‑1 (MQ=255)                                                                       aTGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCt                   <  1:440397/80‑1 (MQ=255)                                                                       aTGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCt                   >  2:440397/1‑80 (MQ=255)                                                                       aTGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTgg         <  2:500058/90‑1 (MQ=255)                                                                        tGGCAACCGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGgt        >  1:165157/1‑90 (MQ=255)                                                                              ccGCTGGGATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTg  >  2:468299/1‑90 (MQ=255)                                                                                     |                                                                                   CTGCAAACAGTAATTATGGTGTTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTG  >  NZ_CP009273/1980336‑1980502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑