| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,051,454 | A→T | intergenic (+399/+54) | yeeN → / ← BW25113_RS10360 | YebC/PmpR family DNA‑binding transcriptional regulator/tRNA‑Asn |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,051,454 | 0 | A | T | 88.9% | 22.5 / ‑2.9 | 9 | intergenic (+399/+54) | yeeN/BW25113_RS10360 | YebC/PmpR family DNA‑binding transcriptional regulator/tRNA‑Asn |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base T (5/3); total (5/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.68e-01 | |||||||||||
ACTGAAGCGTGAGACTCGATTAAGCGCACGAAACACAGAAATCAAAAAACCCGGTCACTTTTTTACAAGGTAACCGGGTAAAAATAATTTTTATTTTTTAACTGTTTTGAGACTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACAT > NZ_CP009273/2051368‑2051540 | aCTGAAGCGTGAGACTCGATTAAGCGCACGAAACACAGAAATCAAAAAACCCGGTCACTTTTTTACAAGGTAACCGGGTAAAAATAattt > 2:482071/1‑90 (MQ=255) aGCGTGAGACTCGATTAAGCGCACGAAACACAGAAATCAAAAAACCCGGTCACTTTTTTACAAGGTAACCGGGTAAAAATAttttttatt < 1:490876/90‑1 (MQ=255) aaGCGCACGAAACACAGAAATCAAAAAACCCGGTCACTTTTTTACAAGGTAACCGGGTAAAAATAATTTTTATTTTTTAACTGTTTTgag < 1:482071/90‑1 (MQ=255) aGCGCACGAAACACAGAAATCAAAAAACCCGGTCACTTTTTTACAAGGTAACCGGGTAAAAATATTTTTTATTTTTTAACTGTTTTgaga < 2:296605/90‑1 (MQ=255) aTCAAAAAACCCGGTCACTTTTTTACAAGGTAACCGGGTAAAAATATTTTTTATTTTTTAACTGTTTTGAGACTCATAGAGATGTCTCaa > 1:297001/1‑90 (MQ=255) aTCAAAAAACCCGGTCACTTTTTTACAAGGTAACCGGGTAAAAATATTTTTTATTTTTTAACTGTTTTGAGACTCATAGAGATGTCTCaa > 2:341133/1‑90 (MQ=255) aTCAAAAAACCCGGTCACTTTTTTACAAGGTAACCGGGTAAAAATATTTTTTATTTTTTAACTGTTTTGAGACTCATAGAGATGTCTCaa > 2:351380/1‑90 (MQ=255) aTCAAAAAACCCGGTCACTTTTTTACAAGGTAACCGGGTAAAAATATTTTTTATTTTTTAACTGTTTTGAGACTCATAGAGATGTCTCaa > 2:477441/1‑90 (MQ=255) aGGTAACCGGGTAAAAATATTTTTTATTCTTTAACTGTTTTGAGACTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGa > 1:469180/1‑90 (MQ=255) gggTAAAAATATTTTTTATTTTTTAACTGTTTTGAGACTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAAcc < 1:429032/90‑1 (MQ=255) gggTAAAAATATTTTTTATTTTTTAACTGTTTTGAGACTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAAcc < 2:297001/90‑1 (MQ=255) atattTTTTATTTTTTAACTGTTTTGAGACTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACAt < 1:55549/90‑1 (MQ=255) | ACTGAAGCGTGAGACTCGATTAAGCGCACGAAACACAGAAATCAAAAAACCCGGTCACTTTTTTACAAGGTAACCGGGTAAAAATAATTTTTATTTTTTAACTGTTTTGAGACTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACAT > NZ_CP009273/2051368‑2051540 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |