| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 232,840 | T→A | V96E (GTG→GAG) | dnaQ → | DNA polymerase III subunit epsilon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 232,840 | 0 | T | A | 92.3% | 41.8 / ‑4.7 | 13 | V96E (GTG→GAG) | dnaQ | DNA polymerase III subunit epsilon |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base A (6/6); total (6/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGA > NZ_CP009273/232753‑232914 | gTATCGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGgaga < 1:379262/90‑1 (MQ=255) ttGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATcc > 1:209787/1‑90 (MQ=255) tGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGc > 1:302513/1‑90 (MQ=255) aaTTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAg < 2:302513/90‑1 (MQ=255) gATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATc < 1:213535/90‑1 (MQ=255) gATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATc < 1:355654/90‑1 (MQ=255) gAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCtt < 2:160585/90‑1 (MQ=255) aTGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAgcgc < 2:180137/90‑1 (MQ=255) tGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGcgcg > 2:216389/1‑90 (MQ=255) aCTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGata < 1:234717/90‑1 (MQ=255) cTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGatat > 2:409635/1‑90 (MQ=255) tataTTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATAtt > 2:297733/1‑90 (MQ=255) ttCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGa < 1:22062/90‑1 (MQ=255) gcggcgcggAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGa > 1:155604/1‑90 (MQ=255) | GTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGA > NZ_CP009273/232753‑232914 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |