| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,922,713 | A→C | T376P (ACC→CCC) | sdaC → | HAAAP family serine/threonine permease SdaC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,922,713 | 0 | A | C | 94.1% | 47.7 / ‑3.2 | 17 | T376P (ACC→CCC) | sdaC | HAAAP family serine/threonine permease SdaC |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (9/7); total (10/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01 | |||||||||||
AACCGTATCACTGCGCTGTTCATGCTGGTAACGACCTGGATTGTTGCCACCCTGAACCCGAGCATCCTGGGTATGATTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTA > NZ_CP009273/2922632‑2922795 | aaCCGTATCACTGCGCTGTTCATGCTGGTAACGACCTGGATTGTTGCCACCCTGAACCCGAGCATCCTGGGTATGATTGAACCCCTGGGc < 1:375076/90‑1 (MQ=255) aCCGTATCACTGCGCTGTTCATGCTGGTAACGACCTGGATTGTTGCCACCCTGAACCCGAGCATCCTGGGTATGATTGAACCCCTGGGCg < 2:452373/90‑1 (MQ=255) aCTGCGCTGTTCATGCTGGTAACGACCTGGATTGTTGCCACCCTGAACCCGAGCATCCTGGGTATGATTGAACCCCTGGGCGGTCCAatc > 2:114336/1‑90 (MQ=255) gcTGTTCATGCTGGTAACGACCTGGATTGTTGCCACCCTGAACCCGAGCATCCTGGGTATGATTGAAACCCTGGGCGGTCCAATCATcgc > 1:285376/1‑90 (MQ=255) gTAACGACCTGGATTGTTGCCACCCTGAACCCGAGCATCCTGGGTATGATTGAACCCCTGGGCGGTCCAATCATCGCGATGATCCTGTTc > 1:416441/1‑90 (MQ=255) tGGAGTGTTGCCACCCTGAACCCGAGCATCCTGGCTATAATTGAACCCCTTCGCGGGCCAATCATCGCGATGACACTGTTCCTCACGCCg > 2:175731/1‑90 (MQ=255) aTTGTTGCCACCCTGAACCCGAGCATCCTGGGTATGATTGAACCCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATg < 1:339772/90‑1 (MQ=255) tGAACCCGAGCATCCTGGGTATGATTGAACCCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGa < 1:364601/90‑1 (MQ=255) gAACCCGAGCATCCTGGGTATGATTGAACCCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGaa > 2:224960/1‑90 (MQ=255) gAACCCGAGCATCCTGGGTATGATTGAACCCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGaa < 1:201485/90‑1 (MQ=255) aaCCCGAGCATCCTGGGTATGATTGAACCCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGaaa < 2:304681/90‑1 (MQ=255) aTGATTGAACCCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAg > 2:451486/1‑90 (MQ=255) tGATTGAACCCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGt > 1:163125/1‑90 (MQ=255) gATTGCACCCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGAGGCAGATGAACGCAATTCAGAAAGTCCCGGCAAGGCGTAAGTa > 2:232549/1‑90 (MQ=255) gATTGAACCCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTa > 2:44963/1‑90 (MQ=255) aTTGAACCCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGaaa > 1:447504/1‑69 (MQ=255) aTTGAACCCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGaaa < 2:447504/69‑1 (MQ=255) | AACCGTATCACTGCGCTGTTCATGCTGGTAACGACCTGGATTGTTGCCACCCTGAACCCGAGCATCCTGGGTATGATTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTA > NZ_CP009273/2922632‑2922795 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |