Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,995,567	A→G	T635A (ACC→GCC)	xdhA →	xanthine dehydrogenase molybdenum‑binding subunit XdhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,995,567	0	A	G	100.0%	107.9 / NA	33	T635A (ACC→GCC)	xdhA	xanthine dehydrogenase molybdenum‑binding subunit XdhA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/10);  total (23/10)

GAAAGCTCCATCAAAACCACCACTAACCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGG  >  NZ_CP009273/2995480‑2995651                                                                                        |                                                                                     gAAAGCTCCATCAAAACCACCACTAACCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCGcc                                                                                    >  1:232720/1‑90 (MQ=255)        ccATCAAAACCACCACTAACCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCGCCATCAAcc                                                                             >  1:27190/1‑90 (MQ=255)        ccATCAAAACCACCACTAACCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCGCCATCAAcc                                                                             >  2:204873/1‑90 (MQ=255)          aTCAAAACCACCACTAACCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCGCCATCAACCGc                                                                           <  1:197628/90‑1 (MQ=255)                acccccACTAACCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCGCCATCAACCGCATCCTc                                                                     <  2:260570/90‑1 (MQ=255)                  caccacTAACCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGCTATTGCGCTGTGCAAAGTCGCCATCAACCGCATCCTCaa                                                                   <  1:401785/90‑1 (MQ=255)                              aCCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTc                                                       >  2:110107/1‑90 (MQ=255)                              aCCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTc                                                       >  2:188279/1‑90 (MQ=255)                                    gTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCa                                                 >  1:433156/1‑90 (MQ=255)                                     tttGGCTGACCCTTTGTTGTTCTCACGGTCGATATTGCGCTGTGCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCat                                                <  2:403301/90‑1 (MQ=255)                                      ttGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCata                                               <  1:156665/90‑1 (MQ=255)                                        ggCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATAtt                                             >  1:32744/1‑90 (MQ=255)                                            gTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTa                                         >  2:374922/1‑90 (MQ=255)                                                 tttGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCa                                    >  1:504550/1‑90 (MQ=255)                                                  ttgttgATCTGACGGTCGATATTGCGCTGTGCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCAc                                   >  1:374702/1‑90 (MQ=255)                                                  ttgttgATCTGACGGTCGATATTGCGCTGTGCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCAc                                   >  1:97558/1‑90 (MQ=255)                                                       gATCTGACGGTCGATATTGCGCTGTGCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCActgctg                              <  2:194297/90‑1 (MQ=255)                                                         tCTGACGGTCGATATTGCGCTGTGCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGc                            >  2:82468/1‑90 (MQ=255)                                                         tCTGACGGTCGATATTGCGCTGTGCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGc                            >  2:344039/1‑90 (MQ=255)                                                             aCGGTCGATATTGCGCTGTGCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGaa                        <  2:374702/90‑1 (MQ=255)                                                               ggTCGATATTGCGCTGTGCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAgg                      <  1:448417/90‑1 (MQ=255)                                                                         gcgcTGTGCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCata                                               <  1:437252/55‑1 (MQ=255)                                                                         gcgcTGTGCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCata                                               >  2:437252/1‑55 (MQ=255)                                                                              gtgCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGTACAcggcgg       >  1:489402/1‑90 (MQ=255)                                                                              gtgCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGTACAcggcgg       >  2:177435/1‑90 (MQ=255)                                                                              gtgCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGTACAcggcgg       >  1:152150/1‑90 (MQ=255)                                                                              gtgCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGTACAcggcgg       >  1:275840/1‑90 (MQ=255)                                                                              gtgCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGTACAcggcgg       >  1:20259/1‑90 (MQ=255)                                                                              gtgCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGTACAcggcgg       >  1:186724/1‑90 (MQ=255)                                                                              gtgCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGTACAcggcgg       >  1:155597/1‑90 (MQ=255)                                                                              gtgCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGCCATATTCTTAATCCACTGCTGGCAGCAGGTCAGGTACAcggcgg       >  2:186506/1‑90 (MQ=255)                                                                                 cAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGTACACGGCggaat    <  2:275840/90‑1 (MQ=255)                                                                                   aaGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGTACACGGCggaatgg  >  1:418201/1‑90 (MQ=255)                                                                                        |                                                                                     GAAAGCTCCATCAAAACCACCACTAACCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGG  >  NZ_CP009273/2995480‑2995651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑