Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,011,010	A→G	E531G (GAA→GGA)	ygfK →	putative selenate reductase subunit YgfK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,011,010	0	A	G	100.0%	73.6 / NA	23	E531G (GAA→GGA)	ygfK	putative selenate reductase subunit YgfK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/9);  total (14/9)

TCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCA  >  NZ_CP009273/3010930‑3011092                                                                                 |                                                                                   ccACCAGTGCAAATACACTTGTCCCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTg                                                                           <  1:391810/89‑1 (MQ=255)  cACCAGTGCCAATACAACTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTgg                                                                          >  1:37766/1‑90 (MQ=255)     cAGTGCCAAAACAACTGTCCCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGAt                                                                       <  1:42438/90‑1 (MQ=255)     cAGGGCAAAAACACCTTTCCCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGAt                                                                       <  1:345615/90‑1 (MQ=255)       gTGCCAATACAACTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGa                                                                     >  2:137762/1‑90 (MQ=255)            aaTACAACTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAAtata                                                                >  1:425900/1‑90 (MQ=255)                     gTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCt                                                       >  1:225461/1‑90 (MQ=255)                     gTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCt                                                       >  1:57708/1‑90 (MQ=255)                          cGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGcac                                                  >  1:32403/1‑90 (MQ=255)                           gCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGcaca                                                 >  1:73662/1‑90 (MQ=255)                               ggATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAGGCAACGCTGGCACAAAcc                                             >  2:339971/1‑90 (MQ=255)                                          gTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTg                                  >  2:431938/1‑90 (MQ=255)                                          gTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTg                                  >  1:492456/1‑90 (MQ=255)                                            gcgcTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGt                                >  1:32485/1‑90 (MQ=255)                                              gcTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTc                              <  1:445097/90‑1 (MQ=255)                                                 gAATATCCGCGAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACg                           <  2:38132/90‑1 (MQ=255)                                                           gAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGtt                 >  1:244824/1‑90 (MQ=255)                                                           gAACTGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGtt                 >  1:393031/1‑90 (MQ=255)                                                               tGAAAAAAGTCGCGCTGGGAAAAGGTTGGGATGAATATAGGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCg             <  1:339971/90‑1 (MQ=255)                                                                 aaaaaaGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTg           <  2:225461/90‑1 (MQ=255)                                                                    aaaGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCACCGCTGGCCCAACCCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGAtt        <  2:318542/90‑1 (MQ=255)                                                                    aaaGTCGCGCTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGAtt        <  1:43946/90‑1 (MQ=255)                                                                          gcgcTGGGAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCa  >  2:321220/1‑90 (MQ=255)                                                                                 |                                                                                   TCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCA  >  NZ_CP009273/3010930‑3011092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑