| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,012,671 | G→C | G51A (GGC→GCC) | ssnA → | putative aminohydrolase SsnA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,012,671 | 0 | G | C | 90.9% | 26.9 / ‑2.6 | 11 | G51A (GGC→GCC) | ssnA | putative aminohydrolase SsnA |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base C (4/6); total (4/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.12e-01 | |||||||||||
ATATCGCCATCGAAAACGATGTGATTGTCGCTATCGGCGATGCCCTGACGCAACGCTACCCCGACGCCAGCTTCAAAGAGATGCATGGCCGGATTGTGATGCCAGGGATTGTCTGCTCGCACAACCATTTTTACTCGGGGCTTTCCCGCGGAATTATGGCAAACATCGC > NZ_CP009273/3012584‑3012752 | atatCGCCATCGAAAACGATGTGATTGTCGCTATCGGCGATGCCCTGACGCAACGCTACCCCGACGCCAGCTTCAAAGAGATGCATGccc < 2:409267/90‑1 (MQ=255)atatCGCCATCGAAAACGATGTGATTGTCGCTATCGGCGATGCCCTGACGCAACGCTACCCCGACGCCAGCTTCAAAGAGATGCATGccc < 2:413757/90‑1 (MQ=255) cGCCATCGAAAACGATGTGATTGTCGCTATCGGCGATGCCCTGACGCAACGCTACCCCGACGCCAGCTTCAAAGAGATGCATGCCCGGAt > 2:139256/1‑90 (MQ=255) cGCCATCGAAAACGATGTGATTGTCGCTATCGGCGATGCCCTGACGCAACGCTACCCCGACGCCAGCTTCAAAGAGATGCATGCCCGGAt > 2:373785/1‑90 (MQ=255) tCGAAAACGATGTGATTGTCGCTATCGGCGATGCCCTGACGCAACGCTACCCCGACGCCAGCTTCAAAGAGATGCATGGCCGGATTGTGa < 2:171495/90‑1 (MQ=255) aTGTGATTGTCGCTATCGGCGATGCCCTGACGCAACGCTACCCCGACGCCAGCTTCAAAGAGATGCATGccc < 1:312795/72‑1 (MQ=255) aTGTGATTGTCGCTATCGGCGATGCCCTGACGCAACGCTACCCCGACGCCAGCTTCAAAGAGATGCATGccc > 2:312795/1‑72 (MQ=255) cGGCGATGCCCTGACGCAACGCTACCCCGACGCCAGCTTCAAAGAGATGCATGCCCGGATTGTGATGCCAGGGATTGTCTGCTCGCACaa < 2:410609/90‑1 (MQ=255) gCGATGCCCTGACGCAACGCTACCCCGACGCCAGCTTCAAAGAGATGCATGCCCGGATTGTGATGCCAGGGATTGTCTGCTCGCACAAcc < 2:144028/90‑1 (MQ=255) gCGATGCCCTGACGCAACGCTACCCCGACGCCAGCTTCAAAGAGATGCATGCCCGGATTGTGATGCCAGGGATTGTCTGCTCGCACAAcc > 2:196982/1‑90 (MQ=255) gACGCAACGCTACCCCGACGCCAGCTTCAAAGAGATGCATGCCCGGATTGTGATGCCAGGGATTGTCTGCTCGCACAACCATTTTTACTc < 1:139256/90‑1 (MQ=255) gACGCAACGCTACCCCGACGCCAGCTTCAAAGAGATGCATGCCCGGATTGTGATGCCAGGGATTGTCTGCTCGCACAACCATTTTTACTc < 1:140217/90‑1 (MQ=255) cccGACGCCAGCTTCAAAGAGATGCATGCCCGGATTGTGATGCCAGGGATTGTCTGCTCGCACAACCATTTTTACTCGGGGCTTTCCcgc < 2:462884/90‑1 (MQ=255) ttCAAAGAGATGCATGCCCGGATTGTGATGCCAGGGATTGTCTGCTCGCACAACCATTTTTACTCGGGGCTTTCCCGCGGAATTATGGCa < 1:268366/90‑1 (MQ=255) gaTGCATGCCCGGATTGTGATGCCAGGGATTGTCTGCTCGCACAACCATTTTTACTCGGGGCTTTCCCGCGGAATTATGGCAAACATCGc > 2:276509/1‑90 (MQ=255) | ATATCGCCATCGAAAACGATGTGATTGTCGCTATCGGCGATGCCCTGACGCAACGCTACCCCGACGCCAGCTTCAAAGAGATGCATGGCCGGATTGTGATGCCAGGGATTGTCTGCTCGCACAACCATTTTTACTCGGGGCTTTCCCGCGGAATTATGGCAAACATCGC > NZ_CP009273/3012584‑3012752 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |