Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,786,991	T→C	D18G (GAT→GGT)	yibB ←	protein YibB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,786,991	0	T	C	100.0%	59.8 / NA	19	D18G (GAT→GGT)	yibB	protein YibB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/12);  total (7/12)

TCAAGTGCGGCCAGACGCTCAAAGTAACTAAAATAAACATCAACTGAACGAGCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAATCCCCTCTACCAATGTCAAAATATGCAGTGATAATTGTGGTGGATGATTTCATTACTAATAAGACCATTAATATAAAAAAAGA  >  NZ_CP009273/3786904‑3787073                                                                                        |                                                                                   tCAAGTGCGGCCAGACGCTCAAAGTAACTAAAATAAACATCAACTGAACGAGCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAAccc                                                                                  <  2:253578/90‑1 (MQ=255)   aaGTGCGGCCAGACGCTCAAAGTAACTAAAATAAACATCAACTGAACGAGCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAACcccc                                                                                <  2:277475/90‑1 (MQ=255)       gCGGCCAGACGCTCAAAGTAACTAAAATAAACATCAACTGAACGAGCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAACCCCCTCTa                                                                            >  1:321083/1‑90 (MQ=255)          gCCAGACGCTCAAAGTAACTAAAATAAACATCAACTGAACGAGCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAACCCCCTCTACCa                                                                         <  2:389555/90‑1 (MQ=255)              gACGCTCAAAGTAACTAAAATAAACATCAACTGAACGAGCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAACCCCCTCTACCAATGt                                                                     <  2:171984/90‑1 (MQ=255)                    cAAAGTAACTAAAATAAACATCAACTGAACGAGCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAACCCCCTCTACCAATGTCAAAat                                                               >  2:410678/1‑90 (MQ=255)                          aaCTAAAATAAACATCAACTGAACGAGCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAACCCCCTCTACCAATGTCAAAATATGCAg                                                         <  1:344986/90‑1 (MQ=255)                            cTAAAATAAACATCAACTGAACGAGCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAACCCCCTCTACCAATGTCAAAATATGCAGTg                                                       <  2:161801/90‑1 (MQ=255)                                 aTAAACATCAACTGAACGAGCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAACCCCCTCTACCAATGTCAAAATATGCAGTGATAAt                                                  <  1:512412/90‑1 (MQ=255)                                      cATCAACTGAACGAGCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAACCCCCTCTACCAATGTCAAAATATGCAGTGATAATTgtgg                                             <  1:461352/90‑1 (MQ=255)                                                    gCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAACCCCCTCTACCAATGTCAAAATATGCa                                                          <  2:255339/63‑1 (MQ=255)                                                    gCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAACCCCCTCTACCAATGTCAAAATATGCa                                                          >  1:255339/1‑63 (MQ=255)                                                           tttCACGGAACCCTTTATTAGCAGTCCAACCCCCTCTACCAATGTCAAAATATGCAGTGATAATTGTGGTGGATGATTTCATTACtaata                        <  1:493671/90‑1 (MQ=255)                                                           tttCACGGAACCCTTTATTAGCAGTCCAACCCCCTCTACCAATGTCAAAATATGCAGTGATAATTGTGGTGGATGATTTCATTACtaata                        <  2:214896/90‑1 (MQ=255)                                                                               gCAGTCCAACCCCCTCTACCAATGTCAAAATATGCa                                                          >  1:377324/1‑36 (MQ=255)                                                                               gCAGTCCAACCCCCTCTACCAATGTCAAAATATGCa                                                          <  2:377324/36‑1 (MQ=39)                                                                                 aGTCCAACCCCCTCTACCAATGTCAAAATATGCAGTGATAATTGTGGTGGATGATTTCATTACTAATAAGACCATTAATATAAAAAAAGa  >  2:243112/1‑90 (MQ=255)                                                                                 aGTCCAACCCCCTCTACCAATGTCAAAATATGCAGTGATAATTGTGGTGGATGATTTCATTACTAATAAGACCATTAATATAAAAAAAGa  >  1:365617/1‑90 (MQ=255)                                                                                  gTCCAACCCCCTCTACCAATGTCAAAATATGCAGTGATAATTGTGGTGgatg                                       <  2:234221/52‑1 (MQ=255)                                                                                  gTCCAACCCCCTCTACCAATGTCAAAATATGCAGTGATAATTGTGGTGgatg                                       >  1:234221/1‑52 (MQ=255)                                                                                        |                                                                                   TCAAGTGCGGCCAGACGCTCAAAGTAACTAAAATAAACATCAACTGAACGAGCTAGTTTTTCACGGAACCCTTTATTAGCAGTCCAATCCCCTCTACCAATGTCAAAATATGCAGTGATAATTGTGGTGGATGATTTCATTACTAATAAGACCATTAATATAAAAAAAGA  >  NZ_CP009273/3786904‑3787073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑