| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,104,266 | G→C | G45G (GGC→GGG) | fpr ← | ferredoxin‑‑NADP(+) reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,104,266 | 0 | G | C | 93.3% | 89.6 / 0.1 | 30 | G45G (GGC→GGG) | fpr | ferredoxin‑‑NADP(+) reductase |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base C (15/13); total (16/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01 | |||||||||||
ATCGGGGACGGTGACCAGGTAAAACTCCAGATCGGGATTATCGGGCGAGTTTACATAGGAGTAGGCGCGCTGGACGCGTTCGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGT > NZ_CP009273/4104185‑4104351 | aTCGGGGACGGTGACCAGGTAAAACTCCAGATCGGGATTATCGGGCGAGTTTACATAGGAGTAGGCGCGCTGGACGCGTTCCCCGTCGAt < 1:314655/90‑1 (MQ=255) gTGACCAGGTAAAACTCCAGATCGGGATTATCGGGCGAGTTTACATAGGAGTAGGCGCGCTGGACGCGTTCCCCGTCGATTTCAAGGCCa < 2:8932/90‑1 (MQ=255) aGGTAAAACTCCAGATCGGGATTATCGGGCGAGTTTACATAGGAGTAGGCGCGCTGGACGCGTTCCCCGTCGATTTCAAGGCCAAGCTTg > 1:265227/1‑90 (MQ=255) ccAGATCGGGATTATCGGGCGAGTTTACATAGGAGTAGGCGCGCTGGACGCGTTCCCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGcc > 1:6037/1‑90 (MQ=255) cAGATCGGGATTATCGGGCGAGTTTACATAGGAGTAGGCGCGCTGGACGCGTTCCCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGccc < 2:403136/90‑1 (MQ=255) cAGATCGGGATTATCGGGCGAGTTTACATAGGAGTAGGCGCGCTGGACGCGTTCCCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGccc < 1:56637/90‑1 (MQ=255) gCGAGTTTACATAGGAGTAGGCGCGCTGGACGCGTTCCCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGccc < 2:519474/73‑1 (MQ=255) gCGAGTTTACATAGGAGTAGGCGCGCTGGACGCGTTCCCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGccc > 1:519474/1‑73 (MQ=255) gCGAGTTTACATAGGAGTAGGCGCGCTGGACGCGTTCCCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCa > 1:151080/1‑90 (MQ=255) aGTTTACATAGGAGTAGGCGCGCTGGACGCGTTCCCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACgg < 1:282821/90‑1 (MQ=255) tACATAGGAGTAGGCGCGCTGGACGCGTTCCCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGc > 1:298011/1‑90 (MQ=255) ggAGTAGGCGCGCTGGACGCGTTCCCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAAc > 2:350406/1‑90 (MQ=255) ggAGTAGGCGCGCTGGACGCGTTCCCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAAc > 1:201801/1‑90 (MQ=255) ggAGAAGGCGCGCTGGACGCGTTCCCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAAc > 1:309899/1‑90 (MQ=255) gAGTAGGCGCGCTGGACGCGTTCCCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACg > 2:52200/1‑90 (MQ=255) gTAGGCGCGCTGGACGCGTTCCCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGt > 1:523360/1‑90 (MQ=255) gTAGGCGCGCTGGACGCGTTCCCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGt < 1:176322/90‑1 (MQ=255) gTAGGCGCGCTGGACGCGTTCCCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGt < 1:101456/90‑1 (MQ=255) aGGCGCGCTGGACGCGTTCCCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTga > 2:63248/1‑90 (MQ=255) aGGCGCGCTGGACGCGTTCCCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTga > 1:130377/1‑90 (MQ=255) aGGCGCGCTGGACGCGTTCCCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTga < 2:309899/90‑1 (MQ=255) aGGCGCGCTGGACGCGTTCCCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTga < 2:21858/90‑1 (MQ=255) cgcgcTGGACGCGTTCCCCGTCGATTTCAAGGCCAGGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGAc > 1:79510/1‑90 (MQ=255) cgcgcTGGACGCGTTCCCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGAc < 2:46856/90‑1 (MQ=255) cgcTGGACGCGTTCCCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTa > 2:472549/1‑90 (MQ=255) gcTGGACGCGTTCGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGa < 2:498140/62‑1 (MQ=255) gcTGGACGCGTTCGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGa > 1:498140/1‑62 (MQ=255) aCGCGTTCCCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAgg < 2:130377/90‑1 (MQ=255) aCGCGTTCCCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAgg < 1:97856/90‑1 (MQ=255) gTTCCCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGt > 2:34551/1‑90 (MQ=255) | ATCGGGGACGGTGACCAGGTAAAACTCCAGATCGGGATTATCGGGCGAGTTTACATAGGAGTAGGCGCGCTGGACGCGTTCGCCGTCGATTTCAAGGCCAAGCTTGGTAAATTGCCCGGCGGTAAACGGAAGCACGGGGGCGTGAACGGTGAGACTAAACAGGGCGT > NZ_CP009273/4104185‑4104351 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |