Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	476,525	A→G	intergenic (‑361/+185)	tomB ← / ← acrB	Hha toxicity modulator TomB/efflux RND transporter permease AcrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	476,525	0	A	G	100.0%	31.4 / NA	11	intergenic (‑361/+185)	tomB/acrB	Hha toxicity modulator TomB/efflux RND transporter permease AcrB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/5);  total (6/5)

ATTGCTCTGAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTAAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTG  >  NZ_CP009273/476442‑476596                                                                                    |                                                                        aTTGCTCTGAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGGttatta                                                                   <  1:304334/90‑1 (MQ=255)     ctctGAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGGTTATTAAATc                                                               <  1:14460/90‑1 (MQ=255)              tGACGTAATAACCGAGGAATGAATAAAGAATTACCGCGAATAATTAAGAATAGCCTCTAAATGATTATGGGTTATTAAATCTATTAgcgc                                                      <  2:407875/90‑1 (MQ=255)              tGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGGTTATTAAATCTATTAgcgc                                                      >  2:208245/1‑90 (MQ=255)                   taataaCCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGGTTATTAAATCTATTAGCGCTACtt                                                 >  2:481219/1‑90 (MQ=255)                        aCCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGGTTAATAAATCTATTAGCGCTACTTAatat                                            >  1:80346/1‑90 (MQ=255)                              gaatgaatAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGGTTATTAAATCTATTAGCGCTACTTAATATAAATta                                      <  1:481219/90‑1 (MQ=255)                                            ttACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGGTTATTAAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTa                        >  2:32553/1‑90 (MQ=255)                                                                tAGCCTCTAAATGATTATGGGTTATTAAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCg    <  1:32553/90‑1 (MQ=255)                                                                tAGCCTCTAAATGATTATGGGTTATTAAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCg    >  2:112257/1‑90 (MQ=255)                                                                  gCCTCTAAATGATTATGGGTTATTAAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTg  >  1:486196/1‑90 (MQ=255)                                                                                    |                                                                        ATTGCTCTGAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTAAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTG  >  NZ_CP009273/476442‑476596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑