Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	661,892	A→C	G594G (GGT→GGG)	mrdA ←	peptidoglycan DD‑transpeptidase MrdA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	661,892	0	A	C	100.0%	55.6 / NA	18	G594G (GGT→GGG)	mrdA	peptidoglycan DD‑transpeptidase MrdA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (12/6);  total (12/6)

CCGCAGGCAGATCGGTGTTGTTATCACCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCACCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTCA  >  NZ_CP009273/661807‑661967                                                                                      |                                                                            ccGCAGGCAGATCGGTGTTGTTATCCCCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGt                                                                         <  2:230403/90‑1 (MQ=255)   gcaggcagATCGGTGTTGTTATCCCCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTtc                                                                       <  2:322833/90‑1 (MQ=255)          gATCGGTGTTGTTATCACCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGaa                                                                >  2:321627/1‑90 (MQ=255)                      tATCACCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAg                                                    >  2:517877/1‑90 (MQ=255)                         cACCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCa                                                 >  2:445158/1‑90 (MQ=255)                             cAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTg                                             >  2:520085/1‑90 (MQ=255)                                  tAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGAt                                        <  1:292921/90‑1 (MQ=255)                                        ggTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATtgttgt                                  >  1:295512/1‑90 (MQ=255)                                         gTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTa                                 >  2:257453/1‑90 (MQ=255)                                         gTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTa                                 >  1:440604/1‑90 (MQ=255)                                                  cTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCGGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCaaa                        >  2:492992/1‑90 (MQ=255)                                                               gtACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGt           >  2:518429/1‑90 (MQ=255)                                                                    aaCCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTtgtg      >  1:91024/1‑90 (MQ=255)                                                                       cgccgGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTc   <  2:40450/90‑1 (MQ=255)                                                                       cgccgGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTc   <  1:316464/90‑1 (MQ=255)                                                                       cgccgGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTc   <  2:456773/90‑1 (MQ=255)                                                                       cgccgGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTc   >  2:84267/1‑90 (MQ=255)                                                                        gccgGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTCa  >  1:158231/1‑90 (MQ=255)                                                                                      |                                                                            CCGCAGGCAGATCGGTGTTGTTATCACCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCACCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTCA  >  NZ_CP009273/661807‑661967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑