Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	979,260	T→C	I52T (ATC→ACC)	gloC →	hydroxyacylglutathione hydrolase GloC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	979,260	0	T	C	100.0%	21.1 / NA	8	I52T (ATC→ACC)	gloC	hydroxyacylglutathione hydrolase GloC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/2);  total (6/2)

GGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGATCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGTGC  >  NZ_CP009273/979199‑979328                                                              |                                                                     ggcggcGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCAc                                          >  1:107647/1‑90 (MQ=255)                        aCAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGgc                   <  2:396686/90‑1 (MQ=255)                                      aCAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCg     >  1:168876/1‑90 (MQ=255)                                         gCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGAcc                                            <  1:299491/48‑1 (MQ=255)                                         gCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGAcc                                            >  2:299491/1‑48 (MQ=255)                                         gCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGTGc  >  2:358079/1‑90 (MQ=255)                                         gCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGTGc  >  2:51881/1‑90 (MQ=255)                                         gCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCACAGCGGCGGAACTGGCGCAACATTACGGCGTGc  >  1:402904/1‑90 (MQ=255)                                                              |                                                                     GGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGATCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGTGC  >  NZ_CP009273/979199‑979328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑