Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,054,234	A→G	E232G (GAA→GGA)	torC →	pentaheme c‑type cytochrome TorC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,054,234	0	A	G	100.0%	26.1 / NA	10	E232G (GAA→GGA)	torC	pentaheme c‑type cytochrome TorC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/3);  total (7/3)

GACACGCTGTACTCTATTGATATTAAGCCAATTTATGCGGCGAAAGGCGATAAAGAAGCCTCTGGTTCTCTGCTGCCTGCTTCGGAAGTGAAAGTCCTTAAACGTGACGGCGACTGGCTGCAAATTGAAATTACCGGCTGGACGGAAAGCGCCGGACGTCAGCGTGTACTC  >  NZ_CP009273/1054149‑1054319                                                                                      |                                                                                      gACACGCTGTACTCTATTGATATTAAGCCAATTTATGCGGCGAAAGGCGATAAAGAAGCCTCTGGTTCTCTGCTGCCTGCTTCGGGAGTg                                                                                   >  1:80250/1‑90 (MQ=255)         gTACTCTATTGATATTAAGCCAATTTATGCGGCGAAAGGCGATAAAGAAGCCTCTGGTTCTCTGCTGCCTGCTTCGGGAGTGAAAGTCCt                                                                           >  1:42533/1‑90 (MQ=255)                   gATATTAAGCCAATTTATGCGGCGAAAGGCGATAAAGAAGCCTCTGGTTCTCTGCTGCCTGCTTCGGGAGTGAAAGTCCTTAAACGTGAc                                                                 >  2:205117/1‑90 (MQ=255)                   gATATTAAGCCAATTTATGCGGCGAAAGGCGATAAAGAAGCCTCTGGTTCTCTGCTGCCTGCTTCGGGAGTGAAAGTCCTTAAACGTGAc                                                                 <  2:65007/90‑1 (MQ=255)                   gATATTAAGCCAATTGATGCGGCGAAAGGCGATAAAGAAGCCTCTGGTTCTCTGCTGCCGGCTTCGGGAGTGAAAGTCCTTAAACGTGAc                                                                 >  2:26241/1‑90 (MQ=255)                     tatTAAGCCAATTTATGCGGCGAAAGGCGATAAAGAAGCCTCTGGTTCTCTGCTGCCTGCTTCGGGAGTGAAAGTCCTTAAACGTGACgg                                                               >  1:286803/1‑90 (MQ=255)                     tatTAAGCCAATTTATGCGGCGAAAGGCGATAAAGAAGCCTCTGGTTCTCTGCTGCCTGCTTCGGGAGTGAAAGTCCTTAAACGTGACgg                                                               >  2:519271/1‑90 (MQ=255)                                        gcgAAAGGCGATAAAGAAGCCTCTGGTTCTCTGCTGCCTGCTTCGGGAGGGAAAGTCCTTAAACGTGACGGCGACTGGCTGCAAATGGaa                                            >  2:304471/1‑90 (MQ=255)                                                                      ctgctgCCTGCTTCGGGAGTGAAAGTCCTTAAACGTGACGGCGACTGGCTGCAAATTGAAATTACCGGCTGGACGGAAAGCGCCGGACGt              <  1:61471/90‑1 (MQ=255)                                                                                  tCGGGAGTGAAAGTCCTTAAACGTGACGGCGACTGGCTGCAAATTGAAATTACCGGCTGGACGGAAAGCGCCGGACGTCAGCGTGTACTc  <  2:286803/90‑1 (MQ=255)                                                                                      |                                                                                      GACACGCTGTACTCTATTGATATTAAGCCAATTTATGCGGCGAAAGGCGATAAAGAAGCCTCTGGTTCTCTGCTGCCTGCTTCGGAAGTGAAAGTCCTTAAACGTGACGGCGACTGGCTGCAAATTGAAATTACCGGCTGGACGGAAAGCGCCGGACGTCAGCGTGTACTC  >  NZ_CP009273/1054149‑1054319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑