Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,065,426	T→G	T129P (ACC→CCC)	rutF ←	pyrimidine utilization flavin reductase protein F

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,065,426	0	T	G	100.0%	53.2 / NA	18	T129P (ACC→CCC)	rutF	pyrimidine utilization flavin reductase protein F
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (14/4);  total (14/4)

GATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCAGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCC  >  NZ_CP009273/1065351‑1065503                                                                            |                                                                              gATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGGGcc                                                                            >  2:57755/1‑79 (MQ=255) gATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGGGcc                                                                            <  1:57755/79‑1 (MQ=255) gATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGGGCCGACGCTcacca                                                                 >  2:321039/1‑90 (MQ=255) gATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGGGCCGACGCTcacca                                                                 >  1:109475/1‑90 (MQ=255)         cacaCCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGGGCCGACGCTCACCACCTGGCTg                                                         >  2:121854/1‑90 (MQ=255)                    gTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGGGCCGACGCTCACCACCTGGCTGATACGGCAGTc                                              >  1:502220/1‑90 (MQ=255)                    gTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGCCGTGGGGGCCGCCGCTCACCACCTGGCTGATACGGCAGTc                                              >  2:505030/1‑90 (MQ=255)                     tAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGGGCCGACGCTCACCACCTGGCTGATACGGCAGTCa                                             >  2:432014/1‑90 (MQ=255)                       gggTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGGGCCGACGCTCACCACCTGGCTGATACGGCAGTCaaa                                           >  2:208990/1‑90 (MQ=255)                         gtgtggtgtgACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGGGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACg                                         >  2:437789/1‑90 (MQ=255)                                  gACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGGGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCagag                                >  2:400397/1‑90 (MQ=255)                                     gATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGGGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCAGAGCct                             >  2:468526/1‑90 (MQ=255)                                        gAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGGGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCAGAGCCTCtt                          >  1:302269/1‑90 (MQ=255)                                                  gATGGCGCAAAACAGAATGTCGTGGGGGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCAGAGCCTCTTCCAGTTGCgg                <  1:208990/90‑1 (MQ=255)                                                     ggCGCAAAACAGAATGTCGTGGGGGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCAGAGCCTCTTCCAGTTGCGGGCa             <  2:363420/90‑1 (MQ=255)                                                                gAATGTCGTGGGGGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCAGAGCCTCTTCCAGTTGCGGGCATCCGGTCAccc  >  1:376982/1‑90 (MQ=255)                                                                     tCGTGGGGGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCAGAGCct                             >  1:325411/1‑58 (MQ=255)                                                                     tCGTGGGGGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCAGAGCct                             <  2:325411/58‑1 (MQ=255)                                                                            |                                                                              GATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCAGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCC  >  NZ_CP009273/1065351‑1065503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑