| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 98,192 | T→C | I314T (ATT→ACT) | murC → | UDP‑N‑acetylmuramate‑‑L‑alanine ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 98,192 | 0 | T | C | 100.0% | 53.1 / NA | 17 | I314T (ATT→ACT) | murC | UDP‑N‑acetylmuramate‑‑L‑alanine ligase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (11/6); total (11/6) | |||||||||||
GAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAGTGAAT > NZ_CP009273/98109‑98271 | gAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCg > 2:174262/1‑90 (MQ=255) tGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGgc < 2:320580/90‑1 (MQ=255) tGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGgc < 2:379895/90‑1 (MQ=255) aTAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTcc < 2:298371/90‑1 (MQ=255) gcTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGAc > 2:158465/1‑90 (MQ=255) gAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTgg > 1:198863/1‑90 (MQ=255) ccgcAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGcc > 2:306108/1‑90 (MQ=255) ccgcAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGcc > 2:417020/1‑90 (MQ=255) cggttgcggttgCTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGAtt < 2:402587/90‑1 (MQ=255) tgcTACGGAAGAGGGCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGa > 1:188909/1‑90 (MQ=255) gggCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAg > 1:42880/1‑46 (MQ=255) gggCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAg < 2:42880/46‑1 (MQ=255) gggCATTGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGa > 2:95726/1‑90 (MQ=255) ttGACGACGAGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAg > 1:517500/1‑90 (MQ=255) cgacgaGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAGTGaa < 1:19868/90‑1 (MQ=255) gacgaGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAGTGAAt > 2:195905/1‑90 (MQ=255) gacgaGGCTACTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAGTGAAt > 2:194100/1‑90 (MQ=255) | GAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCGGTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAGTGAAT > NZ_CP009273/98109‑98271 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |