Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,394,163	G→A	L111L (CTG→TTG)	ogt ←	methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,394,163	0	G	A	100.0%	58.5 / NA	16	L111L (CTG→TTG)	ogt	methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/11);  total (5/11)

GCAAGGTACGACGATGCTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCGTAGCA  >  NZ_CP009273/1394077‑1394248                                                                                       |                                                                                      gCAAGGTACGACGATGCTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGTTCAGCCAATTg                                                                                    <  1:292614/90‑1 (MQ=255)       tACGACGATGCTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTa                                                                              <  1:349284/90‑1 (MQ=255)       tACGACGATGCTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTa                                                                              <  2:25709/90‑1 (MQ=255)       tACGACGATGCTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTa                                                                              <  2:82588/90‑1 (MQ=255)                     tGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCTTAATGCATTACCTGcc                                                                >  1:192242/1‑90 (MQ=255)                        gATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGc                                                             >  2:231610/1‑90 (MQ=255)                          ttCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAg                                                           <  1:28665/90‑1 (MQ=255)                            cGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAggg                                                         <  1:366720/90‑1 (MQ=255)                                ccGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATa                                                     <  2:507738/90‑1 (MQ=255)                                          cACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAgtg                                           <  1:428267/90‑1 (MQ=255)                                                              ccAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGa                       <  1:319533/90‑1 (MQ=255)                                                                aGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGaaa                     <  2:124402/90‑1 (MQ=255)                                                                              gCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCg       >  2:270095/1‑90 (MQ=255)                                                                              gCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCg       >  2:82689/1‑90 (MQ=255)                                                                                tCAGCCAATTGGGCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCGTa     >  2:154646/1‑90 (MQ=255)                                                                                   gCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCGTAGCa  <  2:482258/90‑1 (MQ=255)                                                                                       |                                                                                      GCAAGGTACGACGATGCTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCGTAGCA  >  NZ_CP009273/1394077‑1394248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑