Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,402,000	A→G	I18T (ATC→ACC)	dgcM ←	diguanylate cyclase DgcM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,402,000	0	A	G	100.0%	52.8 / NA	17	I18T (ATC→ACC)	dgcM	diguanylate cyclase DgcM
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/12);  total (5/12)

GCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGATCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGTTGTGCGTAATCATTGAGATCCCGTTATTTTTATCA  >  NZ_CP009273/1401920‑1402074                                                                                 |                                                                           gCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAg                                                                   <  1:415806/90‑1 (MQ=255)    gACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTc                                                                          <  1:197593/80‑1 (MQ=255)    gACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTc                                                                          >  2:197593/1‑80 (MQ=255)    gACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGAc                                                                <  2:300561/90‑1 (MQ=255)            gTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGt                                                        <  1:159728/90‑1 (MQ=255)             tGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTa                                                       <  2:224739/90‑1 (MQ=255)              gAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTaa                                                      >  2:111367/1‑90 (MQ=255)              gAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTaa                                                      >  1:435482/1‑90 (MQ=255)                    ttGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAg                                                <  1:120377/90‑1 (MQ=255)                    ttGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAg                                                <  2:488317/90‑1 (MQ=255)                    ttGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAg                                                <  2:85197/90‑1 (MQ=255)                                 ttCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGTtg                                   <  2:184667/90‑1 (MQ=255)                                      cgcgCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGTTGTGCGt                              <  1:185668/90‑1 (MQ=255)                                      cgcgCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGTTGTGCGt                              <  2:338189/90‑1 (MQ=255)                                                    aaaTTAACTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGTTGTGCGTAATCATTGAGATcc                >  2:137460/1‑90 (MQ=255)                                                         aaCTGTTCCTCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGTTGTGCGTAATCATTGAGATCCCGTTa           >  1:422542/1‑90 (MQ=255)                                                                  tCAAAGGGCGAAACGGTCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGTTGTGCGTAATCATTGAGATCCCGTTATTTTTATCa  <  1:502214/90‑1 (MQ=255)                                                                                 |                                                                           GCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGATCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGTTGTGCGTAATCATTGAGATCCCGTTATTTTTATCA  >  NZ_CP009273/1401920‑1402074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑