Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,494,123	T→C	E195G (GAA→GGA)	ydcK ←	YdcK family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,494,123	0	T	C	100.0%	31.6 / NA	11	E195G (GAA→GGA)	ydcK	YdcK family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/7);  total (4/7)

ATGCCAGCTTTCACCTGCGCGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTTCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCTGG  >  NZ_CP009273/1494048‑1494206                                                                            |                                                                                    aTGCCAGCTTTCACCTGCGCGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCaaaa                                                                       >  1:448149/1‑90 (MQ=255) aTGCCAGCTTTCACCTGCGCGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCaaaa                                                                       >  2:258629/1‑90 (MQ=255)                        aCCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTc                                                <  2:201822/90‑1 (MQ=255)                           gTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAAt                                             <  2:448149/90‑1 (MQ=255)                                            cGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGgacga                            <  1:268774/90‑1 (MQ=255)                                                      gACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGcc                  >  2:480452/1‑90 (MQ=255)                                                         aTTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATa               <  2:247470/90‑1 (MQ=255)                                                            gTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAAt            <  2:102992/90‑1 (MQ=255)                                                            gTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAAt            >  2:6299/1‑90 (MQ=255)                                                                      ttACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCtgg  <  1:480452/90‑1 (MQ=255)                                                                         ccTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCt    <  1:482013/85‑1 (MQ=255)                                                                            |                                                                                    ATGCCAGCTTTCACCTGCGCGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTTCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCTGG  >  NZ_CP009273/1494048‑1494206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑