| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 115,628 | A→C | T137P (ACG→CCG) | ampD → | 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase AmpD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 115,628 | 0 | A | C | 94.4% | 43.0 / ‑5.5 | 18 | T137P (ACG→CCG) | ampD | 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase AmpD |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (9/8); minor base T (1/0); total (10/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
TTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAAC > NZ_CP009273/115549‑115707 | tttttCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACt < 1:143509/90‑1 (MQ=255) aTTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACtgattgat < 1:350529/90‑1 (MQ=255) gggATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGc < 2:105397/90‑1 (MQ=255) tGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTTCGCGGGGACTGATTGATTGCTATcc > 2:308993/1‑90 (MQ=255) tGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCGCTGATTTATTGCTATcc > 1:370360/1‑90 (MQ=255) ttGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGata < 2:237901/90‑1 (MQ=255) gCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTa < 2:66928/90‑1 (MQ=255) ggCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACggg > 1:467268/1‑90 (MQ=255) ggCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCCCGGGGACCGATTGATTGCTATCCCGGTATCGCTAAAAAAATGACggg > 2:336302/1‑90 (MQ=255) tataCCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCAt < 2:467268/90‑1 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTTATTGCTATCCGGATATCGCTAAAAACATGACGGGGCCTTGTGat > 1:307246/1‑90 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCCCGCTGTCGTTTATTGACATCTGGGAATCGCCTAAAACAAGGCGGGCCCTtttggt > 1:380987/1‑88 (MQ=255) tGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGGTTGATTGCTATCCGGGTATCGCTAAAAAAATGACGGGGCATTGTGgtat > 1:471212/1‑90 (MQ=255) gTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCCTTGTGATATTTCGcc > 1:491353/1‑90 (MQ=255) cTTGCGGCGGTTCCCCGGGGACTGATTGATTGCTATCCGGGTATCGCTAAAAACATGACGGGCCCTTGTGGTATTTCGCCGGATCGGgaa > 2:278344/1‑90 (MQ=255) tGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAAc < 2:67450/90‑1 (MQ=255) cggcggTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGAc < 2:364578/55‑1 (MQ=255) cggcggTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGAc > 1:364578/1‑55 (MQ=255) | TTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAAC > NZ_CP009273/115549‑115707 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |