Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,524,815	T→C	intergenic (‑54/‑28)	BW25113_RS25600 ← / → BW25113_RS07655	protein YncO/ISAs1 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,524,815	0	T	C	100.0%	63.7 / NA	20	intergenic (‑54/‑28)	BW25113_RS25600/BW25113_RS07655	protein YncO/ISAs1 family transposase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/12);  total (8/12)

GATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGACAAC  >  NZ_CP009273/1524735‑1524897                                                                                 |                                                                                   gATCATGAAAAATGCGATGTAAATCACGCTTTTCAACTTTGCTATAACGCCTACAGGTGATCCAAATTTAACCTTTGGAACAACTaaaaa                                                                           <  2:517825/90‑1 (MQ=255)     aTGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATa                                                                       >  1:295845/1‑90 (MQ=255)            ttGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAgg                                                                <  1:420425/90‑1 (MQ=255)                 aTGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGcc                                                           <  1:510141/90‑1 (MQ=255)                   gTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAg                                                         >  2:127797/1‑90 (MQ=255)                        tCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAg                                                    <  1:157861/90‑1 (MQ=255)                         cACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGt                                                   <  2:295845/90‑1 (MQ=255)                         cACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGt                                                   <  2:155528/90‑1 (MQ=255)                             aTTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTc                                               <  1:314361/90‑1 (MQ=255)                             aTTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTc                                               >  2:118047/1‑90 (MQ=255)                                       ttGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCt                                     >  1:409289/1‑90 (MQ=255)                                                   cTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTc                         >  1:31376/1‑90 (MQ=255)                                                       aGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTg                     <  2:54845/90‑1 (MQ=255)                                                       aGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTg                     <  1:264506/90‑1 (MQ=255)                                                       aGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTg                     <  1:261675/90‑1 (MQ=255)                                                           gATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGAccc                 >  1:75201/1‑90 (MQ=255)                                                            aTCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGAcccc                >  1:341577/1‑90 (MQ=255)                                                             tCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCt               <  2:298837/90‑1 (MQ=255)                                                                          tttGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGacaac  <  1:287919/90‑1 (MQ=255)                                                                          tttGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGacaac  >  1:191228/1‑90 (MQ=255)                                                                                 |                                                                                   GATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGACAAC  >  NZ_CP009273/1524735‑1524897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑