Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,560,820	T→C	E193G (GAA→GGA)	dosC ←	diguanylate cyclase DosC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,560,820	0	T	C	100.0%	35.7 / NA	12	E193G (GAA→GGA)	dosC	diguanylate cyclase DosC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/5);  total (7/5)

CACAGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTTCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGAGAAGATACGATAGTT  >  NZ_CP009273/1560739‑1560896                                                                                  |                                                                             cacaGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTCCCCATGaa                                                                      <  2:522551/90‑1 (MQ=255)   caGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTCCCCATGAAAg                                                                    >  2:172669/1‑90 (MQ=255)               aGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAAATAATATCTATTCCCCATGAAAGTATTGAGGCTAt                                                        >  2:509407/1‑90 (MQ=255)                       ttAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATAATCTCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGtt                                                <  1:289073/90‑1 (MQ=255)                       ttAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGtt                                                <  1:220784/90‑1 (MQ=255)                       ttAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGtt                                                <  1:327809/90‑1 (MQ=255)                                         cTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGt                              >  1:127877/1‑90 (MQ=255)                                                 gAATCCAGCAGGATTTTATAGATAATATCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGt                      >  2:407828/1‑90 (MQ=255)                                                 gAATCCAGCAGGATTTTATAGATAATATCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGt                      >  2:69125/1‑90 (MQ=255)                                                       agcagGATTTTATAGATAATATCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACgag                <  1:427403/90‑1 (MQ=255)                                                           ggATTTTATAGATAATATCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGagaaga            >  2:431035/1‑90 (MQ=255)                                                                     gATAATATCTATTCCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGAGAAGATACGATAGtt  >  1:203731/1‑90 (MQ=255)                                                                                  |                                                                             CACAGGCCAAAATCAGCCTGGCTTAAAGGCAAACTACTGCCTAAATCAGAATCCAGCAGGATTTTATAGATAATATCTATTTCCCATGAAAGTATTGAGGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGAGAAGATACGATAGTT  >  NZ_CP009273/1560739‑1560896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑