Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,573,157	A→T	I148N (ATC→AAC)	yddA ←	ABC transporter ATP‑binding protein/permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,573,157	0	A	T	100.0%	97.1 / NA	26	I148N (ATC→AAC)	yddA	ABC transporter ATP‑binding protein/permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (9/17);  total (9/17)

CCGCGCTTTCCCAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGA  >  NZ_CP009273/1573071‑1573232                                                                                       |                                                                            ccGCGCTTTCCCAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTc                                                                           <  2:50071/89‑1 (MQ=255) ccGCGCTTTCCCAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTc                                                                           >  1:50071/1‑89 (MQ=255)          cccAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGaa                                                                 <  2:422385/90‑1 (MQ=255)                  aTAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCa                                                         >  2:36052/1‑90 (MQ=255)                    aaCGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGc                                                       >  1:480711/1‑90 (MQ=255)                          aaaGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACg                                                 >  2:49296/1‑90 (MQ=255)                           aaGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGt                                                <  2:498826/90‑1 (MQ=255)                               tGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGt                                            <  1:524348/90‑1 (MQ=255)                               tGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGt                                            <  1:36052/90‑1 (MQ=255)                                  tCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAg                                         <  1:302296/90‑1 (MQ=255)                                       aTGCTAAGTGTCTGGATGAAGCCAAATGAAAGACTGGGCTTTTTGTTTTTCAGCAAAAGAATGTCTCCAGGGATCCTTTGGTCAGGTTTa                                    >  2:238943/1‑90 (MQ=255)                                       aTGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATATGTTGGTCAGGGTTa                                    <  2:359430/90‑1 (MQ=255)                                       aTGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTa                                    >  1:403421/1‑90 (MQ=255)                                       aTGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTa                                    >  2:157664/1‑90 (MQ=255)                                         gCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATc                                  <  1:139707/90‑1 (MQ=255)                                         gCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATc                                  <  1:16164/90‑1 (MQ=255)                                                      aTGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGc                     <  1:248365/90‑1 (MQ=255)                                                        gAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTc                   <  2:219916/90‑1 (MQ=255)                                                        gAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTc                   <  2:249781/90‑1 (MQ=255)                                                        gAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTc                   >  1:431917/1‑90 (MQ=255)                                                        gAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTc                   <  1:169772/90‑1 (MQ=255)                                                                  gAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATc         <  2:175065/90‑1 (MQ=255)                                                                       aCTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGt    <  1:368406/90‑1 (MQ=255)                                                                         tGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGa  <  1:313348/90‑1 (MQ=255)                                                                          gAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGa                                                     >  2:276568/1‑38 (MQ=38)                                                                          gAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGa                                                     <  1:276568/38‑1 (MQ=38)                                                                                       |                                                                            CCGCGCTTTCCCAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGA  >  NZ_CP009273/1573071‑1573232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑