Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,782,276	T→G	V192G (GTG→GGG)	ppsR →	posphoenolpyruvate synthetase regulatory kinase/phosphorylase PpsR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,782,276	0	T	G	100.0%	31.5 / NA	11	V192G (GTG→GGG)	ppsR	posphoenolpyruvate synthetase regulatory kinase/phosphorylase PpsR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (9/2);  total (9/2)

CCACCAGTCTGTATCTGGCAATGCAGTTTGGTATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGTGCTACCCGCGTCGCTCAAACCGCTTCAGCATAAATTGTTCGGCCTGACTATCGACCCGGAACGTCTGGCGGCGATTCGCGAG  >  NZ_CP009273/1782195‑1782358                                                                                  |                                                                                   ccaccaGTCTGTATCTGGCAATGCAGTTTGGTATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGGGCTACCcg                                                                            >  2:301272/1‑90 (MQ=255)                 ggCAATGCAGTTTGGTATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGGGCTACCCGCGTCGCTCAAACCGCt                                                            >  1:395764/1‑90 (MQ=255)                 ggCAATGCAGTTTGGTATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGGGCTACCCGCGTCGCTCAAACCGCt                                                            >  2:220603/1‑90 (MQ=255)                     aTGCAGTTTGGTATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGGGCTACCCGCGTCGCTCAAACCGCTTCAg                                                        >  1:325455/1‑90 (MQ=255)                     aTGCAGTTTGGTATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGGGCTACCCGCGTCGCTCAAACCGCTTCAg                                                        >  1:80138/1‑90 (MQ=255)                     aTGCAGTTTGGTATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGGGCTACCCGCGTCGCTCAAACCGCTTCAg                                                        >  2:64799/1‑90 (MQ=255)                                                           tGCCGACGATATGGATAATCTGGGGCTACCCGCGTCGCTCAAACCGCTTCAGCATAAATTGTTCGGCCTGACTATCGACCCGGAACGTCt                  >  2:297357/1‑90 (MQ=255)                                                               gacgaTATGGATAATCTGGGGCTACCCGCGTCGCTCAAACCGCTTCAGCACAAATTGTTCGGCCTGACTATCGACCCGGAACGTCTggcg              >  1:376416/1‑90 (MQ=255)                                                                    tatGGATAATCTGGGGCTACCCGCGTCGCTCAAACCGCTTCAGCATAAATTGTTCGGCCTGACTATCGACCCGGAACGTCTGGCGGCGAt         <  1:220603/90‑1 (MQ=255)                                                                           aaTCTGGGGCTACCCGCGTCGCTCAAACCGCTTCAGCATAAATTGTTCGGCCTGACTATCGACCCGGAACGTCTGGCGGCGATTCGCGAg  >  1:122957/1‑90 (MQ=255)                                                                           aaTCTGGGGCTACCCGCGTCGCTCAAACCGCTTCAGCACAAATTGTTCGGCCTGACTATCGACCCGGAACGTCTGGCGGCGATTCGCGAg  <  2:376416/90‑1 (MQ=255)                                                                                  |                                                                                   CCACCAGTCTGTATCTGGCAATGCAGTTTGGTATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGTGCTACCCGCGTCGCTCAAACCGCTTCAGCATAAATTGTTCGGCCTGACTATCGACCCGGAACGTCTGGCGGCGATTCGCGAG  >  NZ_CP009273/1782195‑1782358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑