Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,898,828 (T)5→4 coding (130/1878 nt) bglF ← PTS beta‑glucoside transporter subunit IIABC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,898,8240T.100.0% 46.7 / NA 12coding (134/1878 nt)bglFPTS beta‑glucoside transporter subunit IIABC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (6/6);  total (6/6)

ACCGCCAGGAAGACATCGGCCACATGGTTACCTATGACCACCTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATG  >  NZ_CP009273/3898737‑3898907
                                                                                       |                                                                                   
aCCGCCAGGAAGACATCGGCCACATGGTTACCTATGACCACCTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCttt                                                                                   <  1:218530/90‑1 (MQ=255)
    ccAGGAAGACATCGGCCACATGGTTACCTATGACCACCTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCttttcag                                                                               >  1:108095/1‑87 (MQ=255)
                      caTGGTTACCTATGACCACCTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTCAGTACCTCTGCTTGCgcttt                                                            <  2:165240/90‑1 (MQ=255)
                              ccTATGACCACCTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCAt                                                    <  2:5153/90‑1 (MQ=255)
                                       accTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCtttaatt                                           >  1:320992/1‑90 (MQ=255)
                                                  gCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAAt                                >  1:98721/1‑90 (MQ=255)
                                                            tCCACCACCATAATAATACCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTgcgc                      <  1:327280/90‑1 (MQ=255)
                                                                           ataCCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTca       <  2:308092/90‑1 (MQ=255)
                                                                             aCCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTcaca     <  1:63378/90‑1 (MQ=255)
                                                                                gggggTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTca       >  1:335286/1‑85 (MQ=255)
                                                                                gggggTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTca       <  2:335286/85‑1 (MQ=255)
                                                                                gggggTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATg  >  1:56815/1‑90 (MQ=255)
                                                                                gggggTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATg  >  2:90593/1‑90 (MQ=255)
                                                                                gggggTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATg  >  2:90866/1‑90 (MQ=255)
                                                                                       |                                                                                   
ACCGCCAGGAAGACATCGGCCACATGGTTACCTATGACCACCTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATG  >  NZ_CP009273/3898737‑3898907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: