Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 1,076,727 (T)5→4 intergenic (+458/‑85) putP → / → BW25113_RS05295 sodium/proline symporter PutP/FTR1 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,076,7230T.100.0% 42.4 / NA 11intergenic (+454/‑89)putP/BW25113_RS05295sodium/proline symporter PutP/FTR1 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (7/4);  total (7/4)

CTGGAATAATCGTTTATATCCCTTGGCATTACCTCTCTTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTTCTTTGCCGTAATGATAATCGCTATCACTGCGATTTACTTTTCTTTGCATAGATTGACTCAGAAAAACGTTTAAGGGTGG  >  NZ_CP009273/1076643‑1076806
                                                                                |                                                                                   
cTGGAATAATCGTTTATATCCCTTGGCATTACCTCTCTTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTCTTTGc                                                                           >  2:64032/1‑90 (MQ=255)
       aatCGTTTATATCCCTTGGCATTACCTCTCTTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTCTTTGCCg                                                                         >  1:328846/1‑85 (MQ=255)
       aatCGTTTATATCCCTTGGCATTACCTCTCTTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTCTTTGCCg                                                                         <  2:328846/85‑1 (MQ=255)
            tttATATCCCTTGGCATTACCTCTCTTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTCTTTGCCGTAATGATAAt                                                               >  1:511/1‑90 (MQ=255)
                      ttGGCATTACCTCTCTTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTCTTTGCCGTAATGATAATCGCTATCACt                                                     >  2:131937/1‑90 (MQ=255)
                                 tctctTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTCTTTGCCGTAATGATAATCGCTATCACTGCGATTTACtt                                          <  2:511/90‑1 (MQ=255)
                                      ttGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTCTTTGCCGTAATGATAATCGCTATCACTGCGATTTACTTTTCtt                                     <  1:200528/90‑1 (MQ=255)
                                         tttACATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTCTTTGCCGTAATGATAATCGCTATCACTGCGATTTACTTTTCTTTGc                                  >  1:103892/1‑90 (MQ=255)
                                           tACATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTCTTTGCCGTAATGATAATCGCTATCACTGCGATTTACTTTTCTTTGCAt                                <  2:34269/90‑1 (MQ=255)
                                            aCATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTCTTTGCCGTAATGATAATCGCTATCACTGCGATTTACTTTTCTTTGCATa                               >  1:293833/1‑90 (MQ=255)
                                                                         gCTTGTGTTTTCTTTGCCGTAATGATAATCGCTATCACTGCGATTTACTTTTCTTTGCATAGATTGACTCAGAAAAACGTTTAAgggtgg  >  1:285408/1‑90 (MQ=255)
                                                                                |                                                                                   
CTGGAATAATCGTTTATATCCCTTGGCATTACCTCTCTTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTTCTTTGCCGTAATGATAATCGCTATCACTGCGATTTACTTTTCTTTGCATAGATTGACTCAGAAAAACGTTTAAGGGTGG  >  NZ_CP009273/1076643‑1076806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: