Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,122,270	(A)6→5	coding (9/924 nt)	yceM →	Gfo/Idh/MocA family oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,122,265	0	A	.	100.0%	19.9 / NA	6	coding (4/924 nt)	yceM	Gfo/Idh/MocA family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (2/4);  total (2/4)

GGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGA  >  NZ_CP009273/1122179‑1122349                                                                                       |                                                                                     ggCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGaaaa                                                                                   >  2:268961/1‑90 (MQ=255)      gcgTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGaaaaattac                                                                              <  2:90119/90‑5 (MQ=255)           gAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATc                                                                        <  2:15814/90‑1 (MQ=255)             aGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCgg                                                                      <  1:270543/90‑1 (MQ=255)                  tGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAg                                                                 >  2:195123/1‑90 (MQ=255)                                       tGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTgcgc                                            >  2:261371/1‑90 (MQ=255)                                                                   ccATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTggcggc                <  1:268961/90‑1 (MQ=255)                                                                                 aaaGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGa  <  1:173653/90‑1 (MQ=255)                                                                                       |                                                                                     GGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGA  >  NZ_CP009273/1122179‑1122349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑