Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,276,251	Δ1 bp	coding (932/3744 nt)	narG →	nitrate reductase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,276,249	0	G	.	100.0%	24.3 / NA	7	coding (930/3744 nt)	narG	nitrate reductase subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (1/6);  total (1/6)

CACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGAC  >  NZ_CP009273/1276162‑1276327                                                                                        |                                                                               cacaCCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATggc                                                                              <  2:135542/90‑2 (MQ=255)                          aaaCTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAT‑GGCCACGTAATGCTGCGTGAATTCCAcc                                                    <  2:133215/90‑1 (MQ=255)                                    aTCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAT‑GGCCACGTAATGCTGCGTGAATTCCACCTCGACAAccc                                          <  1:115700/90‑1 (MQ=255)                                      cTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAT‑GGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCaa                                        >  1:107308/1‑90 (MQ=255)                                                     aaaCAGGGCACCGATGCGGCAATGGCGCTGGCGAT‑GGCCCCGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCg                         <  1:268504/90‑1 (MQ=255)                                                            gCACCGATGCGGCAATGGCGCTGGCGAT‑GGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTAtgt                  <  2:107308/90‑1 (MQ=255)                                                              aCCGATGCGGCAATGGCGCTGGCGAT‑GGCCACGTAATGCTGCGTGAATTCCCCCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGc                <  2:276411/90‑1 (MQ=255)                                                                            ggCGCTGGCGAT‑GGCCACGTAATGCTGCGTGAATTCCCCCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGAc  <  1:149934/90‑1 (MQ=255)                                                                                        |                                                                               CACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGAC  >  NZ_CP009273/1276162‑1276327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑