Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 2,061,342 T→A intergenic (‑576/‑774) BW25113_RS10415 ← / → BW25113_RS24005 IS5‑like element IS5 family transposase/TonB‑dependent receptor plug domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,061,3420TA100.0% 42.5 / NA 12intergenic (‑576/‑774)BW25113_RS10415/BW25113_RS24005IS5‑like element IS5 family transposase/TonB‑dependent receptor plug domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (6/6);  total (6/6)

CTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAATACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGAAGCTA  >  NZ_CP009273/2061267‑2061417
                                                                           |                                                                           
cTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAATACGAAGTTGCTCAGGAACATTAGTGGTGCTg                                                               <  1:164342/90‑1 (MQ=255)
    tctcAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAGTACGAAGTTGCTCAGGAACATTAGTGGTGCTGAGaa                                                           <  2:121526/90‑1 (MQ=255)
               gCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAATACGAAGTTGCTCAGGAACATTAGTGGTGCTGAGAAGTTAAATAAcc                                                >  1:326294/1‑90 (MQ=255)
                      cAGACACAGCTTTCTACTCCATGGTAATGGCCCAAATACGAAGTTGCTCAGGAACATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGa                                         <  2:347778/90‑1 (MQ=255)
                        gACACAGCTTTCTACTCCATGGTAATGGCCCAAATACGAAGTTGCTCAGGAACATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGt                                       <  1:145934/90‑1 (MQ=255)
                        gACACAGCTTTCTACTCCATGGTAATGGCCCAAATACGAAGTTGCTCAGGAACATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGt                                       <  1:314666/90‑1 (MQ=255)
                            caGCTTTCTACTCCATGGTAATGGCCCAAATACGAAGTTGCTCAGGAACATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCa                                   >  2:346072/1‑90 (MQ=255)
                                        ccATGGTAATGGCCCAAATACGAAGTTGCTCAGGAACATTAGTGGTGCTGAGAAGTTAAATAACCATGc                                            >  1:342119/1‑69 (MQ=255)
                                        ccATGGTAATGGCCCAAATACGAAGTTGCTCAGGAACATTAGTGGTGCTGAGAAGTTAAATAACCATGc                                            <  2:342119/69‑1 (MQ=255)
                                                       aaaTACGAAGTTGCTCAGGAACATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATg        >  2:153051/1‑90 (MQ=255)
                                                             gAAGTTGCTCAGGAACATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGAAGCTa  >  1:253392/1‑90 (MQ=255)
                                                             gAAGTTGCTCAGGAACATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGAAGCTa  >  2:243451/1‑90 (MQ=255)
                                                                           |                                                                           
CTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAATACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGAAGCTA  >  NZ_CP009273/2061267‑2061417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: