Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,197,981	T→C	intergenic (+213/‑94)	BW25113_RS11050 → / → yehL	hypothetical protein/MoxR family ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,197,981	0	T	C	100.0%	28.9 / NA	10	intergenic (+213/‑94)	BW25113_RS11050/yehL	hypothetical protein/MoxR family ATPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/6);  total (4/6)

GTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTATTTCATTAATACATTTCAG  >  NZ_CP009273/2197911‑2198064                                                                       |                                                                                    gtTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTCTCCAGATATTTTTCGCTCa                                                                  >  2:64103/1‑90 (MQ=255)  ttAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTCTCCAGATATTTTTCGCTCaa                                                                 >  2:197818/1‑90 (MQ=255)        cTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTCTCCAGATATTTTTCGCTCAAACAACt                                                           >  2:110963/1‑90 (MQ=255)                  taaataaaAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTCTCCAGATATTTTTCGCTCAAACAACTAATGCGCCaa                                                 <  1:32089/90‑1 (MQ=255)                             aCCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTCTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTATTgc                                      >  2:128186/1‑90 (MQ=255)                                                ttttttATTTACTGTGATCTGCTCTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTAtt                   <  1:315615/90‑1 (MQ=255)                                                ttttttATTTACTGTGATCTGCTCTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTAtt                   <  2:187301/90‑1 (MQ=255)                                                  ttttATTTACTGTGATCTGCTCTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTATTTc                 <  1:110963/90‑1 (MQ=255)                                                          aCTGTGATCTGCTCTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTATTTCATTAATAc         <  1:197818/90‑1 (MQ=255)                                                                 tCTGCTCTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTATTTCATTAATACATTTCAg  <  1:331366/90‑1 (MQ=255)                                                                       |                                                                                    GTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTATTTCATTAATACATTTCAG  >  NZ_CP009273/2197911‑2198064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑