Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	232,840	T→A	V96E (GTG→GAG)	dnaQ →	DNA polymerase III subunit epsilon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	232,840	0	T	A	100.0%	29.1 / NA	9	V96E (GTG→GAG)	dnaQ	DNA polymerase III subunit epsilon
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (3/6);  total (3/6)

GTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTT  >  NZ_CP009273/232753‑232923                                                                                        |                                                                                    gTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTTATGGACTATATTCGCGGCGCGGAGTTGgaga                                                                                   <  2:113619/90‑1 (MQ=255) gTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGgaga                                                                                   <  2:31401/90‑1 (MQ=255)             aaTTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAg                                                                       <  1:321249/90‑1 (MQ=255)                       cGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGatat                                                             >  2:132310/1‑90 (MQ=255)                                                       aGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTa                             <  2:344795/90‑1 (MQ=255)                                                            aTGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAgcgc                        <  1:211051/90‑1 (MQ=255)                                                              ggACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGa                      <  1:89788/90‑1 (MQ=255)                                                                  tataTTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATAtt                  >  1:207181/1‑90 (MQ=255)                                                                  tataTTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATAtt                  >  2:117834/1‑90 (MQ=255)                                                                        cgcggcgcggAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAg            <  2:69561/90‑1 (MQ=255)                                                                                  aGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACtt  <  2:197466/90‑1 (MQ=255)                                                                                        |                                                                                    GTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTT  >  NZ_CP009273/232753‑232923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑