Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,591,366	C→T	D287N (GAC→AAC)	bamC ←	outer membrane protein assembly factor BamC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,591,366	0	C	T	100.0%	45.5 / NA	13	D287N (GAC→AAC)	bamC	outer membrane protein assembly factor BamC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/1);  total (12/1)

CTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGC  >  NZ_CP009273/2591281‑2591451                                                                                      |                                                                                      ctgctgCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTTGCTg                                                                                   >  2:190301/1‑90 (MQ=255)    ctgcGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTTGCTGTCa                                                                                >  1:192043/1‑90 (MQ=255)    ctgcGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTTGCTGTCa                                                                                >  2:293918/1‑90 (MQ=255)          ttATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTTGCTGTCAGACAGc                                                                          <  1:305523/90‑1 (MQ=255)          ttATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTTGCTGTCAGACAGc                                                                          >  2:213023/1‑90 (MQ=255)                     gCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTTGCTGTCAGACAGCGGCTTATAAGt                                                               >  2:313329/1‑90 (MQ=255)                                          aCCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTTGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGa                                          >  2:335010/1‑90 (MQ=255)                                          aCCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTTGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGa                                          >  2:81147/1‑90 (MQ=255)                                                   cAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTTGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCt                                 >  2:274372/1‑90 (MQ=255)                                                        cTGGATCGCTCGCGCCCAGTTCCTGCCAGTTGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTACCCTGCGAACGGGTGCTGTCgg                            >  2:79685/1‑90 (MQ=255)                                                               gctcgcGCCCAGTTCCTGCCAGTTGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCTCTGCGAACGGGTGCTGTCGGTCACttt                     >  1:93584/1‑90 (MQ=255)                                                               gctcgcGCCCAGTTCCTGCCAGTTGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACttt                     >  2:209669/1‑90 (MQ=255)                                                                                  cAGTTGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAgc  >  1:272566/1‑90 (MQ=255)                                                                                      |                                                                                      CTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGC  >  NZ_CP009273/2591281‑2591451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑